摘要
双光子显微镜是一种对活体组织进行荧光成像的新技术。它由于具有对细胞损伤小、成像深度大和可用于活细胞长时间观察等优点而逐渐被应用于细胞生物学、神经生物学、免疫学等研究领域。近些年来双光子显微镜在免疫学研究中的应用很广，主要是用于免疫细胞发育、分化、迁移和功能研究等方面。
Two-photon microscopy is a new technology for fluorescence imaging of living tissues. Be- cause it produses less damage to ceils and deeper imaging degree. It can be used to observe living cells for longer time. For these reasons, it has been gradually applied to cell biology, neurobiology, immunology and other research fields. This paper reviews the application of two-photon microscopy in immunology, especially in the study of the development, differentiation, migration and function of immune cells.
出处
《国际免疫学杂志》
CAS
2012年第6期411-415,共5页
International Journal of Immunology
基金
国家自然科学基金（81072405）
关键词
双光子显微镜
免疫学
活体成像
Two-photoa microscopy
Immunology
Intravital imaging
