大孔树脂纯化红松松球鳞片多酚及其抗氧化活性研究

Purification technology of polyphenol from Korean pine cone lamella with macroporous resins and research of their antioxidant ability

通过吸附和解吸实验,从树脂ADS-7、S-8、NKA-9、NKA-Ⅱ、HPD600、AB-8、X-5、D101、D3520中筛选出了适合纯化红松松球鳞片多酚的大孔树脂,并确定纯化工艺参数。结果显示,AB-8树脂为吸附分离红松松球鳞片多酚类物质的优良材料,纯化工艺条件为:上样体积为0.3BV,上样浓度为1.5mg/mL,上样后静态吸附3h,水洗3BV,洗脱剂为90%乙醇,洗脱剂用量为1.6BV。在此条件下,多酚的纯度由12.51%提高到35.07%。同时对纯化前后的抗氧化活性进行了比较。结果表明,红松松球鳞片多酚经纯化后还原Fe3+的能力和清除DPPH自由基的能力都强于粗提物。

According to adsorption and desorption property of macroporous resins to polyphenol,an optimal macroporous resin was chosen from ADS-7、S-8、NKA-9、NKA-Ⅱ、HPD600、AB-8、X-5、D101、D3520 resins for purifying polyphenol from Korean pine cone lamella.AB-8 was found to be the most appropriate resin.The purification of polyphenol was studied,optimum conditions were as follows：the loaded amount was 0.3BV,the concentration of polyphenol was 1.5mg/mL,the time static absorption was 3h,the volume of elution water was 3BV,the eluting solvent was 90% alcohol,the volume of alcohol was 1.6BV.Under these conditions,the purity of polyphenol was 12.51% in the purified samples,while it was 35.07% in the crude samples.In addition,the comparative study on the antioxidant ability was performed between crude and purified samples.The result showed that Fe3＋ reducing power and the scavenging activity on DPPH radical were enhanced after the samples were purified.