果糖月桂酸单酯的分离纯化及鉴定

Purification and identification of monolauroyl fructose

采用硅胶柱层析色谱对叔戊醇中固定化磷脂酶Lecitase Ultra催化合成的果糖月桂酸单酯进行分离纯化。确定了薄层层析(TLC)条件,并通过反相-高效液相色谱-蒸发光散射检测(RP-HPLC-ELSD)测定柱层析纯化产物的纯度。利用液质联用(HPLC-ESI-MS)、红外光谱(FT-IR)和13C核磁共振(13CNMR)对目标产物进行确认,鉴定目标产物为酯化位点在1位和6位的果糖月桂酸单酯,四种异构体如下:1-月桂酸-β-D-吡喃果糖单酯、1-月桂酸-α-D-吡喃果糖单酯、1-月桂酸-β-D-呋喃果糖单酯和6-月桂酸-β-D-呋喃果糖单酯,且1-月桂酸-β-D-吡喃果糖单酯的比例最高。

A novel enzymatic-catalyzing process of monolauroyl fructose was developed.In the process immobilized Lecitase Ultra was used as biocatalyst in anhydrous 2-methyl-2-butanol.Silica gel column chromatography was used to separate and purify the fructose ester.The qualitative analysis condition of TLC was confirmed and the purity was identified by RP-HPLC-ELSD.Structure was verified by HPLC-ESI-MS,FT-IR,13C NMR.As a result,a mixture of the C-1 and C-6 monoacylated frutose esters,four isomers of monolauroyl fructose were obtained：l-lauroyl-β-D-fructopyranose,1-lauroyl-α-D-fructopyranose,1-lauroyl-β-D-fructofuranose,6-lauroyl-β-D-fructofuranose,and l-lauroyl-β-D-fructopyranose was the highest proportion.