败酱草提取物体内抗氧化活性研究

Antioxidant activities in vivo of the extract from Patrinia

目的:研究败酱草提取物对小鼠体内抗氧化作用。方法:采用颈肩部皮下注射D-半乳糖生理盐水无菌溶液制造衰老模型,在正常饲料中分别加入不同含量败酱草提取物,做成低、中、高和最高四个剂量组。8周后摘眼球取血及脱臼处死、组织取样,用试剂盒测定小鼠血清、肝脏、脑组织中MDA含量、SOD、GSH-PX活性及血清、肝脏中CAT活性。结果:败酱草提取物可显著降低血清及组织MDA含量,提高SOD、GSH-PX和CAT活力。结论:败酱草提取物具有显著的体内抗氧化活性。

Objective：Extractive of patrinia were detected for its antioxidant effect in mice.Methods：D-galactose saline sterile solution was injected in shoulder and neck subcutaneous.After that the aging model was manufactured.Producted four dose groups which were low,medium,high and top by adding different amounts of patrinia extracted in four groups of ordinary fodder.Different fodder were fed mices.After eight weeks,researchers produced the test sample by getting blood from the six group mice after excising eyes,dislocating executed,tissuing sampling.Then the MDA content,the GSH-PX and SOD activity in the serum,liver and brain fissure were measured.At the same time the CAT activity in the liver and serum were measured Results：The role of Patriniace extract：Significantly reduced the MDA content in serum and tissues in mices.Improved the activity of SOD,GSH-PX and CAT in mices.Conclusion：Patriniace extract had the significant antioxidant activity in vivo.