大豆低聚肽降胆固醇作用研究

Cholesterol-lowering effect of soybean oligopeptides

目的:研究大豆低聚肽在降胆固醇方面的作用。方法:取5周龄SD雄性大鼠90只,随机分为6组(每组15只),即Ⅰ(对照组)、Ⅱ(蛋白组)、Ⅲ(多肽组)、Ⅳ(低聚肽组1)、Ⅴ(低聚肽组2)、Ⅵ(低聚肽组3),在实验环境下,构建高胆固醇模型,在对各组继续饲喂高胆固醇饲料的同时,对第Ⅱ组口腔灌喂大豆蛋白,对第Ⅲ组口腔灌喂大豆多肽,对第Ⅳ、Ⅴ、Ⅵ组分别灌喂不同剂量的大豆低聚肽,第Ⅰ组口腔灌喂同体积的饮用水。饲喂6周,分别收集粪便、血清和肝脏,测定血清和肝脏中的胆固醇和甘油三酯含量、粪便重量及其中的类固醇含量。结果:第Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ组血清和肝脏中的胆固醇和甘油三酯含量均有不同程度的降低,大鼠粪便的排泄量及粪便中类固醇的含量增加。大豆低聚肽组有剂量依赖性,高剂量大豆低聚肽组效果极显著。结论:大豆低聚肽具有比大豆蛋白和大豆多肽更强的降低胆固醇的作用,且这种作用具有剂量依赖性。

Objective：Cholesterol-lowering effect of soybean oligopeptides were studied.Method：In the experimental environment,90 male SD rats at 5 weeks old had been randomly divided into 6 groups,Ⅰ（control group）,II（protein）,III（polypeptides）,IV（oligopeptides group 1）,V（oligopeptides group 2） and VI（oligopeptides group 3）,high cholesterol model of these rats had been constructed at first,then the high cholesterol had been used to each group,at the same time,soy protein had been used to group II,soybean polypeptide had been used to group III,different doses of soy oligopeptides had been given to group IV,V and VI,drinking water with the same volume had been given to group I.The stools,blood serums and livers were collected and triglyceride contents,fecal weight and the steroid content were determined after 6 weeks.Results：The cholesterol and triglyceride content in serum and liver were reduced at different levels in group II,III,IV,V and VI,and the defecation and fecal steroid content of those groups were increased.Those results in group IV,V and VI were dose dependently,and the cholesterol-lowering effect was significantly in the group with the highest dose.Conclusion：Cholesterol-lowering effects of soybean oligopeptides were stronger than soybean protein and polypeptide,and this effect was dose-dependently.