两步酶法合成MLM型结构脂质中醇解反应研究

Optimization of Alcoholysis Reaction for Two-Step Enzymatic Synthesis of MLM-Type Structural Lipid

为获得纯度较高的2-单甘酯来制备MLM型结构脂质,以菜籽油和无水乙醇为底物,用来自Thermomyceslanuginosa固定化脂肪酶TL IM作为催化酶来制备2-单甘酯。在单因素试验的基础上,采用正交试验设计,获得醇解反应的最佳工艺条件:菜籽油/无水乙醇(底物)物质的量比1:5,Lipozyme TL IM酶加入量为7%(以底物质量计)、反应温度35℃、反应时间18h,总单甘酯含量为38.82%。经三级分子蒸馏纯化后,总单甘酯的纯度可以达到90.76%。

In order to obtain monoglyceride（MAG） with high purity for the synthesis of MLM-type structure lipid,immobilized lipase TL IM from Thermomyces lanuginose was used to catalyze the synthesis of MAG by alcoholysis of rapeseed oil and anhydrous ethanol.The optimal alcoholysis conditions were determined by one-factor-at-a-time and orthogonal array design as follows： rapeseed oil/anhydrous ethanol molar ratio of 1：5,lipase dosage of 7%（m/m,on the basis of the weight of substrates）,reaction temperature of 35 ℃ and reaction time of 18 h,resulting in an MAG content of 38.82% in the final products.The purity of the MAG obtained could be increased to 90.76% by a three-stage molecular distillation process.