高效液相色谱法检测三两半药酒中阿魏酸

Determination of Ferulic Acid in Sanliangban Medicinal Liquor by HPLC

目的:建立一种高效液相色谱法测定三两半药酒中阿魏酸含量的方法。方法:采用高效液相色谱,波长316nm、柱温25℃、流速1.0mL/min、进样量20μL;从不同品牌色谱柱、流动相类型及组成比例3个方面筛选阿魏酸检测条件;从线性范围、回收率、精密度、重现性及稳定性等方面进行考察。结果:以EclipseXDB-C18(250mm×4.60mm,5μm)为色谱柱,乙腈-0.1%磷酸(15:85,V/V)为流动相检测效果良好。该方法在1.15～114.70μg/mL内线性关系良好(R2=0.9999),进样精密度(RSD)为0.4%(n=6),进样重现性RSD为1.2%(n=6×2),平均回收率为99.5%,RSD为0.5%(n=6×2),在18h内检测8次,RSD为1.4%。结论:本法简便、准确、专属性强,重现性好,可用于该药酒质量控制。

Objective： To develop an HPLC method for determining ferulic acid in Sanliangban medicinal liquor.Methods： At a flow rate of 1.0 mL/min,the prepared analyte was injected at a level of 20 μL for detection at 316 nm.The column temperature was 25 ℃.Efforts were made to determine optimal column type and mobile phase composition.Moreover,the developed HPLC method was investigated in terms of its linear range,recovery rate,precision,reproducibility and stability.Results： Eclipse XDB-C18column（250 mm × 4.60 mm,5 μm） was the best column for separation of ferulic acid.Good separation was achieved using a mobile phase made up of acetonitrile and 0.1% phosphoric acid（15：85,V/V）.The calibration curve for ferulic acid was linear within the range of 1.15–114.70 μg/mL（R2 = 0.9999）.The precision（RSD）,reproducibility（RSD）,recovery rate and stability（RSD） of this method were 0.4%（n = 6）,1.2%（n = 6 × 2）,99.5% and 0.5%（n = 6 × 2）,respectively.The RSD for 8 repeated determinations within 18 h was 1.4%.Conclusion： This method is simple,accurate,specific,reproducible and applicable for quality control of Sanliangban medicinal liquor.