口腔诊疗环境清洁消毒效果的研究

Effect of cleaning and disinfection in dental treatment room

目的了解北京地区口腔诊疗环境现状,探讨提高口腔诊疗环境清洁消毒效果的方法及监测手段。方法采用三磷酸腺苷(ATP)生物荧光检测法监测分层随机抽取的125所口腔医疗机构环境表面清洁现状,采用平行对照研究比较卡瓦布和乙醇棉的清洁消毒效果,分析菌落计数法与ATP生物荧光法数值的相关性。结果采用ATP方法对医师侧操作台面、医师侧灯把手、患者左侧扶手分别对125份标本进行检测,医师侧操作台面合格率为45.6%;卡瓦布和乙醇棉经菌落计数法检测消毒合格率达100.0%,经ATP生物荧光检测法检测,合格率为50.0%;ATP法测定数值(RLU)与菌落计数法所计算的菌落形成单位(CFU)无明确相关性。结论卡瓦布可以有效用于物体表面清洁消毒,口腔诊疗环境清洁状况需要加强监管,ATP生物荧光检测法可以作为快速监测的手段。

OBJECTIVE To identify the contamination status of general surface in dental treatment room in Beijing and to research on the disinfection and monitoring methods to improve the effect of environment cleaning.METHODS By means of ATP bioluminescence method,the contamination of object surfaces in 125 stratified randomly samples from Beijing dental clinics was evaluated.A parallel control study was conducted to compare the effect of alcohol gauze and CaviWipes.The correlation between the detected data of ATP bioluminescence method and plate culture method was analyzed.RESULTS With ATP bioluminescence method,among 125 groups of dental unit surface samples including light handle aside dentist,panel plate aside dentist and left armrest,the qualified rate in panel plate aside dentist was 45.6%.With plate culture method,the qualified rate of alcohol gauze and CaviWipes was 100.0%.The lg RLU of bioluminescence method was not significantly associated with the lgCFU of plate-culture method.CONCLUSION CaviWipes can be effectively used in the dental clinic surface clean and disinfection.The environment sanity of dental clinics should be supervised and ATP bioluminescence can be used as a quick monitoring method.