冰片对真菌细胞超微结构的影响及治疗化脓性中耳炎的临床应用

Observation of Cell Ultrastructuse in Suppurative Otitis Media Treated with Bosneol and Application

目的 :研究冰片治疗化脓性中耳炎的药理作用 ,最佳有效浓度。方法 :取患者外耳道内分泌物中分离出的黑曲菌和实验室保留的真菌菌株 ,测定冰片最低抑真菌浓度 (MIC) ,最低杀真菌浓度 (MFC)。电子显微镜下观察冰片作用后的细胞超微结构变化。结果 :发现冰片对真菌MIC为 5 % ,MFC为 10 %。电镜下见冰片作用后的黑曲菌细胞壁变厚 ,模糊不清有空泡。细胞浆内各种物质深染凝集。呈现大量脂滴。电子密度增高 ,嵴排列紊乱。有的细胞器破坏 ,呈现大量电子空白区。有的细胞扭曲变形失去原有结构。结论 :冰片可破坏真菌细胞的结构 ,导致真菌溶解死亡 ,表现出抑菌杀菌功能。最低抑真菌浓度为 5 % ,杀菌浓度为 10 %。

Objective:To explore the pharmacological action and optimal concentration of borneol in treating suppurative otitis media.Method:Aspergillus niger separated from the secretion in external auditory canal of the patient and the fungus strains kept in the laboratory were used to determine the minimum fungus inhibiting concentration (MIC) and minimum fungus killing concent ration (MFC)of borned.The change of cell ultrastructure after borneol taking effect was observed under electron microscope.Result:Borneol MIC and MFC turned out to be 5% and 10% respectively.The cell wall of Aspergillus niger became thicker and blurred with vacuoles.In the cytoplasm various materials were found deep dyed and agglutinated with many fat drops.The electron density increased and the ridge arrangement was in disorder. Some organelles were destroyed,and many areas of electron blank appeared.Some cells became warped and deformed and lost their original structure.Conclusion:Borneol can destroy the structure of fungus cells,make fungus dissolve and dieout and thus features a fungus inhibiting and killing function.The optimal inhibiting concentration is 5%and killing concentration 10%.