急性肾功能衰竭与内皮素-1关系的电镜免疫组化观察

Observation of immunohistochemistry and immunoelectron microscopy of ET 1 in acute renal failure

目的 :探讨 ET- 1在 ARF发病机制中的作用。方法 :甘油肌注法复制成大鼠 ARF模型 ,免疫电镜技术动态观察 ET- 1在超微水平的分布与变化。结果 :ARF大鼠肾小管上皮及其线粒体中 ET- 1含量明显增加 ,且与肾组织病变程度呈正相关。结论 :肾小管上皮细胞中 ET- 1持续增多是导致肾小管坏死的重要原因 ,提示 ET-

Objective:To explore the effects of Endothelin 1 (ET 1) on acute renal failure (ARF). Methods:A model of ARF was done by injecting glycerol into the muscle of Wistar rats. We utilized immnohistochemistry and immunoelectronmicroscopy method at ultrastructure level in observation of the distribution and content of ET 1 in kidney of ARF rats. Results:ET 1 content in ARF rats was increased, most of ET 1 particles were distributed with plasma and mitochondria of renal tubular epithelial cells and at same time there were serious pathology changes. Conclusion:The maintain increase of ET 1 content is an important cause of renal tubular necrosis in ARF. It is suggested that ET 1 take part in the mechanism of ARF.