变应性鼻炎患者外周血中IL-27和Th17细胞的相关性

Association between IL-27 and Th17 Cells in Peripheral Blood of Patients with Allergic Rhinitis

目的探讨变应性鼻炎(allergic rhinitis,AR)患者外周血中IL-27、IL-17和Th17细胞的表达及相关性。方法 2012年4月至6月收集变应原为尘螨的变应性鼻炎患者18例(男性10例,女性8例)为AR组,无变态反应疾病的健康志愿者10例(男性4例,女性6例)为对照组,采用ELISA法检测两组外周血的血清中IL-27和IL-17的水平,流式细胞术检测外周血中Th17细胞的百分率。结果 AR组和对照组IL-27的水平分别为(21.69±12.62)pg/mL和(53.10±12.55)pg/mL,两组比较差异有统计学意义(P<0.01);AR组和对照组IL-17的水平分别为(672.82±63.45)pg/mL和(576.62±22.81)pg/mL,两组比较差异有统计学意义(P<0.01);AR组Th17细胞百分率为1.76%±0.60%,对照组为0.59%±0.17%,两组比较差异有统计学意义(P<0.01)。IL-27和Th17细胞、IL-17呈负相关性(r分别为-0.361、-0.435,P<0.05)。结论变应性鼻炎患者外周血中IL-27水平降低,Th17细胞百分率及IL-17水平升高,且IL-27和Th17细胞、IL-17呈负相关,提示IL-27对Th17细胞的抑制功能下降在变应性鼻炎发病机制中可能起着重要的作用。

Objective To explore the expression and association oflL-27, IL-17 and Thl7 cells in peripheral blood of patients with allergic rhinitis （allergic rhinitis, AR）. Methods 18 cases of allergic rhinitis patients （10 males, 8 females） whose allergen was dust mite were collected flom April to June 2012 as AR group, and 10 cases of healthy volunteers （4 males, 6 females） without allergic diseases were put into control group. IL-27 and IL-17 levels in serum of peripheral blood of the two groups were detected by ELISA, and the percentage of Thl 7 cell was detected by flow cytometry. Results IL-27 levels of AR group and control group were （21.69±12.62） pg/mL and （53.10±12.55） pg/mL respectively, and the difference was statistically significant （P〈0.01）; IL-17 levels of AR group and control group were （672.82± 63.45） pg/mL and （576.62±22.81） pg/mL respectively, and the difference was statistically significant （P 〈0.01）; Thl7 cell percentage of AR group was 1.76%±0.60%, and in control group it was 0.59% ±0.17%, with statistically significant difference between the two groups （P 〈0.01）. IL-27 was negatively correlated to Thl7 cell and 1L-17 （r was -0.361 and -0.435 respectively, P 〈0.05）. Conclusions The reduction of IL-27 level in the peripheral blood of patients with allergic rhinitis, the increase of Thl7 cells percentage and 1L-17 level, as well as the negative correlation of IL-27 with Thl7 cell and IL-17 suggest that decline of IL-27 suppression to Thl7 cell may play an important role in the pathogenesis of allergic rhinitis.