气管上皮嗜银细胞和5-羟色胺免疫反应细胞的定位

Localization of Argyrophil Cells and 5-HT Immunoreactive Cells in the Trachea of Several Animals

用银染法及免疫组织化学PAP法 ,对狗、家兔、豚鼠、大鼠、小鼠气管粘膜上皮内嗜银细胞和5-羟色胺 (5-HT)免疫反应 (immunoreactionIR)细胞的分布及形态进行观察。结果显示 :5种动物气管上皮中均可见散在的嗜银细胞 ,家兔气管内嗜银细胞密度较大 ,而狗、大鼠的较少。 5-HT-IR细胞仅见家兔、豚鼠、小鼠的气管粘膜上皮。气管中的内分泌细胞可分为开放型和闭合型 ,并可见旁突伸至邻近细胞。相邻切片观察未见气管的内分泌细胞中有嗜银颗粒与 5-HT共存的现象。

The distribution and morphology of argyrophil cells and 5-HT immunoreactive cells in the trachea of dog,rabbit,guinea pig,rat and mouse were studied with double silver staining method and immunohistochemical PAP method.The results showed that the argyrophil cells appeared scattering in the tracheal epthelium of five kinds of animals. The density of argyrophil cells in the tracheal was high in the rabbit and low in the dog and rat. The 5-HT immunoreactive cells were found in the tracheal epithelium of rabbit,guinea pig and mouse. The open or close type endocrine cells could be distinguished and the cells processes could be seen extending to adjacent cells. As compared with adjacent sections, the co-localization of 5-HT and argyrophil particle in the same cells was not found. The significance about the results as above was discussed in this paper.