金标渗滤法快速检测血吸虫循环抗原的现场应用研究

THE FIELD TRIAL ON RAPID DETECTION OF SCHISTOSOMA CIRCULATING ANTIGENS WITH DOT IMMUNOGOLD FILTRATION ASSAY (DIGFA)

将抗重组蛋白SVLBP-IgG用于DIGFA检测2221例血清中血吸虫循环抗原，其中急性血吸虫病人血清70份，慢性血吸虫病人血清307份，循环抗原检出率分别为100％和68．4％。非流行区健康人血清200份，未见阳性反应。除肺吸虫外，与其它寄生虫成非寄生虫感染病人血清无明显交叉反应。用DIGFA单盲法检测2批血清，结果显示对急性血吸虫病人的敏感性均为100％；对慢性血吸虫病人的敏感性分别为69．4％和68．9％，非流行区体检人群血清的阴性率为99％和98．9％。检测血吸虫病流行区人群血清508份，阳性检出率为20．9％（106／508）；粪检阳性65例，与粪检符合率为70．8％。上述结果提示，该法用于植测循环抗原具有较高的敏感性和特异性，可用于现场查病和流行病学调查。

A total number of 2221 sera collected from different kinds of subjects,i.e.patients with various stages of schistosomiasis,nomal individuals,persons with other parasitic diseases or non-parasitic diseases,were detected with Dot Immunogold Filtration Assay (DIGFA) using anti-SVLBP IgG as capturing antibody.Positive rate of 70 sera from patients with acute schistosomiasis was 100%, and the of 307 sera with chronic schistosomiasis was 68.4% .None of 200 sera from normal indriduals showed false positive reactions.No obvious cross reaction was found in sera forom other parasitic/non-parasitic diseases except sera from patients with paragonimiasis.2 batches of sera were detected with DIGFA by single-blindness method.Results showed that sensitivities to chronic schistosomiasis were 69.4% and 68.9%, and 100% to acute schistosomiasis while specificities were 98.9%-99% .508 samples from residents in endemic areas were tested with DIGFA and Kato-Katz.Positive rate of circulating antigen was 20.9% with DIGFA while 65 samples were positive with stool examination.46 samples were positive both by DIGFA and by Kato-Katz.The coincidental rate was 70.8% .These results indicated that DIGFA showed sensitive and specific in the detection of circulating antigens.It will be useful in mass application and valuable in epidemiological survey.