摘要
将抗重组蛋白SVLBP-IgG用于DIGFA检测2221例血清中血吸虫循环抗原,其中急性血吸虫病人血清70份,慢性血吸虫病人血清307份,循环抗原检出率分别为100%和68.4%。非流行区健康人血清200份,未见阳性反应。除肺吸虫外,与其它寄生虫成非寄生虫感染病人血清无明显交叉反应。用DIGFA单盲法检测2批血清,结果显示对急性血吸虫病人的敏感性均为100%;对慢性血吸虫病人的敏感性分别为69.4%和68.9%,非流行区体检人群血清的阴性率为99%和98.9%。检测血吸虫病流行区人群血清508份,阳性检出率为20.9%(106/508);粪检阳性65例,与粪检符合率为70.8%。上述结果提示,该法用于植测循环抗原具有较高的敏感性和特异性,可用于现场查病和流行病学调查。
A total number of 2221 sera collected from different kinds of subjects,i.e.patients with various stages of schistosomiasis,nomal individuals,persons with other parasitic diseases or non-parasitic diseases,were detected with Dot Immunogold Filtration Assay (DIGFA) using anti-SVLBP IgG as capturing antibody.Positive rate of 70 sera from patients with acute schistosomiasis was 100%, and the of 307 sera with chronic schistosomiasis was 68.4% .None of 200 sera from normal indriduals showed false positive reactions.No obvious cross reaction was found in sera forom other parasitic/non-parasitic diseases except sera from patients with paragonimiasis.2 batches of sera were detected with DIGFA by single-blindness method.Results showed that sensitivities to chronic schistosomiasis were 69.4% and 68.9%, and 100% to acute schistosomiasis while specificities were 98.9%-99% .508 samples from residents in endemic areas were tested with DIGFA and Kato-Katz.Positive rate of circulating antigen was 20.9% with DIGFA while 65 samples were positive with stool examination.46 samples were positive both by DIGFA and by Kato-Katz.The coincidental rate was 70.8% .These results indicated that DIGFA showed sensitive and specific in the detection of circulating antigens.It will be useful in mass application and valuable in epidemiological survey.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第2期19-20,共2页
Chinese Journal of Zoonoses