恶性疟原虫FCC1/HN株CSP抗原细胞免疫应答IL-2和IFN-γ的体外诱生分析

T CELL IMMUNE RESPONSE TO THE CIRCUMSPOROZOITE PROTEIN OF PLASMODIUM FALCIPARUM,SOUTHERN CHINA ISOLATE FCC/HN AND ANTIGEN-INDUCED IL-2 AND IFN-γ Production ANALYSIS OF SPECIFIC

本实验利用恶性疟原虫FCC1／HN株CSP抗原基因在真核细胞（HeLa）中高效表达，将纯化的表达蛋白作为候选疫苗免疫小鼠，并通过体外诱生检测小鼠脾细胞白介素2（IL-2）和γ干扰素（IFN-γ）水平。结果显示经疫苗免疫后的BALB／C小鼠脾细胞体外经特异性刺激后产生的IL-2和IFN-γ水平有显著提高。与我们前期研究结果比较，表明对该疫苗特异性的IL-2、IFN-γ的产生与淋巴细胞增殖以及NK细胞活性增高有明显的相关性；该疫苗对T细胞有一定的刺激能力，可诱导小鼠产生较强的细胞免疫反应。

In this study, the DNA fragment encoding the regions Ⅰto Ⅱ of CSP gene from Plasmodium falciparun isolate FCC1/HN was cloned into an expression vector pcDNA3 contained cytomegalovirus (CMV) and transformed into human Hela cell line.The expressed protein PfCSP (condidatevaccine) was used to immunize BALB/c mice by subcutaneous inrtavenous or intraperitoneal administration respectively.The splenocyte of BALB/c mice immunized with the condidate vaccine released significantly IL-2 and IFN-γ following stimulation wiht this vaccine It is associated with increase of the splenic T lymphocyte proliferation stimulated by this vaccine and enhancement of NK cell killingactivity in the former studies.These results suggested that the vaccine could stimulate T cell response and enhance the cell-mediated immunity.