原位杂交检测喉癌中金葡菌L型DNA

IN SITU BY BRIDIZATION DETECTING L-FORM DNA OF STAPHYLOCOCCUS AUREUS OF LARYNGOCARCINOMA

目的探讨喉癌细胞中是否有金葡菌L型DNA原位杂交阳性表达。方法应用原位核酸杂交技术检测20例喉癌细胞核内金葡菌L型DNA的表达。结果60％癌细胞核、浆内显示金葡菌L型DNA阳性信号。免疫组化染色（S—P法）金葡菌L型抗原阳性率为75％。革兰氏染色金葡菌L型阳性率为70％。结论金葡菌L型DNA已进入喉癌细胞内，极有可能与宿主细胞DNA整合，从基因水平上影响细胞增生和癌变，提示金葡菌及其L型感染与喉癌发生和发展可能有密切的关系[6]。

Aim To study whether there is positive express in situ hybridization of L-form DNA or Staphylococcus aureus in larygocarcinoma cell.Methods 20 cases of expressinon of L-form DNA of Staphylococcus aureus in laryngocarcinoma karyon were detected with nucleic acid in situ hybridization.Results 60% cancer karyon, cytoplasm display positive signal of Staphylococcus aureus L-form DNA positive rate of Staphylococcus aureus L-from antigen is 75% with immunohischemical (s-p) staining.The rate is 70% with gram staining.Conclusion Staphylococcus aureus L-from DNA had get into laryngocarcinoma cells, is great possibly to integrate into host cell,and influence hyperplasis and canceration of cell from gene level. Staphylococcus aureus and its L-form infection have possibly close relation to happen and development of laryngocarcinoma.