1,6_二磷酸果糖钙盐对家犬的药代动力学研究

Pharmacokinetic Study of Fluctose_1,6_ diphosphate Calcium in Dogs

目的 :家犬口服FDP钙盐 2g kg或静脉输注 10 0、2 0 0mg kg(速率 2mL min、4mL min)观察其吸收情况及药物动力学参数。方法 :用Michal和Beutler氏酶法检测血浆FDP浓度 ,用CAPP软件确定模型和拟合C_T曲线。结果 :c_t曲线为二室开放模型 ,静脉输注药动学参数分别为 :β(min-1) 0 .113± 0 .0 76和 0 .118± 0 .0 55;t1 2 β(min) 6 .6 2± 2 .95和 6 .96± 3.0 3;Tmax(min) 1.4 6± 0 .4 6和 1.90± 0 .2 9;Cmax(μg mL) 144 .6 2± 94 .58和 2 52 .4 6± 73.2 1;AUC(mg L·min-1) 781.4 7± 2 81.97和 1974 .11± 797.2 1。Cmax和AUC是随剂量增大而增大 ,其余参数两种剂量间无显著性差异 (P >0 .0 5)。口服FDP钙盐胃肠道吸收有限 ,本文对其原因作了探讨。

Aim:The absorption and pharmacokinetics of fluctose_1,6_diphosphate calcium(FDP) were studied by 2 g/kg oral administration and 100,200 mg/kg intravenous infusion (rate 2 mL/min -1 ,4 mL/min -1 ) in 23 dogs. Method: The plasma levels of FDP were measured by Michal and Beutler′s enzyme assay. The concentration_time curves was fitted to a two_compartment open model by CAPP software. Results: The major pharmacokinetic parameters of the two dosages FDP calcium intravenous infusion were shown as following: C max (μg/mL)144.62±94.58 and 252.46±73.21, T max (min)1.46±0.46 and 1.90±0.29, t 1/2β (min)6.62±2.95 and 6.96±3.03 AUC(mg/L·min -1 )781.47±281.97 and 1974.11±797.21 respectively. C max and AUC were increased with dosage. Other parameters were no significance between the two dosages. The limited absorption of FDP has been showed by oral administration. The reason for its poor absorption from gastrointestinal tract was discussed in the paper.