二苯乙烯苷对H_2O_2诱导血管内皮细胞ICAM-1及VCAM-1表达的影响

Effect of TSG on Expression of ICAM-1 and VCAM-1 Induced by H_2O_2 on Vascular Endothelial Cell

目的:通过研究二苯乙烯苷(TSG)对H2O2诱导的人脐静脉内皮细胞ICAM-1、VCAM-1表达的影响,探明二苯乙烯苷抗氧化保护内皮细胞的作用机制。方法:体外培养人脐静脉内皮细胞,实验分为空白对照组、H2O2组、辛伐他汀组、TSG组,运用逆转录聚合酶链式反应和酶联免疫吸附试验分别检测ICAM-1及VCAM-1 mRNA与其蛋白的表达。结果:200μmol.L-1的H2O2作用内皮细胞24 h后,ICAM-1和VCAM-1的mRNA和蛋白表达水平均明显上调,与空白对照组比较,差异有显著性(P<0.01)。而在200μmol.L-1的H2O2作用前用1μmol.L-1二苯乙烯苷预处理体外培养人脐静脉内皮细胞4 h,结果显示二苯乙烯苷能抑制H2O2诱导的内皮细胞ICAM-1、VCAM-1的mRNA和VCAM-1的蛋白水平表达,与H2O2组比较差异有显著性(P<0.01);而ICAM-1的蛋白表达水平与H2O2组比较差异有统计学意义(P<0.05);辛伐他汀组ICAM-1和VCAM-1的mRNA及其蛋白水平表达降低,与H2O2组比较差异均有显著性(P<0.01)。实验结果表明二苯乙烯苷可抑制H2O2诱导的内皮细胞粘附分子ICAM-1、VCAM-1表达。结论:二苯乙烯苷可通过降低细胞粘附分子ICAM-1和VCAM-1的表达保护氧化应激引起的人脐静脉内皮细胞损伤。

Objective： To study the effect of 2,3,5,4＇-tetrahydroxystilbene-2-O-β -D-glucoside on expression of adhesion molecules ICAM-1, VCAM-1 induced by H202 on human umbilical vein endothelial cells. Methods： The cultured cells were divided into four groups： control group, H202 group, positive group, and TSG group. The expression of ICAM-1 and VCAM-1 mRNA and protein were detected by reverse transcriptase polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbent assay （ELISA） respectively. Results：Treated endothelial cells with 200 μmol-L-1 H2O2, the expression of ICAM-I and VCAM-1 mRNA and protein level was significant higher （P〈0.01）. TSG pretreatment endothelial cells for 4h before treated with 200 μmol. L-1 H2O2, results showed that TSG could inhibit the expression of ICAM-1 and VCAM-1 mRNA and protein level induced by H202 on endothelial cells. Conclusions： TSG can inhibit the expression of adhesion molecules induced by H202 on human umbilical vein endothelial cells.