摘要
目的:建立舒新片中丹皮酚的含量测定方法。方法:用高效液相色谱法对舒新片中丹皮酚进行定量分析,色谱柱为KromsalC18柱(250 mm×4.6 mm,5μm);流动相为甲醇-水(60∶40);流速:1.0mL/min;检测波长274nm。结果 :丹皮酚在0.021～0.105mg/ml范围内线性关系良好,r=0.9997;平均加样回收率为99.07%,RSD为0.36%。结论:该方法简单、准确、专属性强、重复性好,可有效控制舒新片中丹皮酚的含量。
Objective： To establish the content determination method of paeonol in Shuxin Pills. Methods： The quantitative analysis of paeonol in Shuxin Pills was established by HPLC with Kromsal C18 chromatographic column （250mm× 4.6mm, 5μm）, mobile phase methanol-water （60：40）, 1.0 mL/min flow rate, 274nm detection wavelength. Results： The linear ranges were 0.021-0.105 mg/ml with good positive correlation, r was 0.9997. The average recovery rate was 99.07% and RSD was 0.36%. Conclusion： The established methods are accurate, exclusive, reproducible and suitable for the quality control of paeonol in Shuxin Pills.
出处
《现代生物医学进展》
CAS
2012年第30期5930-5932,共3页
Progress in Modern Biomedicine
基金
陕西省中医药管理局资助项目(zy38)
