新风胶囊对佐剂性关节炎大鼠心功能及心肌超微结构影响

Effects of Xinfeng Capsule on the Cardiac Function and the Myocardial Ultrastructure in Rats with Adjuvant Arthritis

目的观察佐剂性关节炎(adjuvant arthritis,AA)大鼠心功能、心肌超微结构变化及新风胶囊对其影响。方法将60只大鼠随机分为正常组、模型组、甲氨喋呤组、雷公藤多苷片组和新风胶囊组,每组12只,除正常组外,分别对其余各组大鼠的右后足跖皮内注射弗氏完全佐剂致炎,致炎后第19天开始给药。正常组及模型组均给予生理盐水,其余3组分别给予甲氨喋呤、雷公藤多苷片和新风胶囊,给药30天。观察各组大鼠足跖肿胀度、关节炎指数(arthritis index,AI)、心功能、血清细胞因子及心肌超微结构变化。结果 (1)与正常组比较,模型组及给药3组给药前大鼠足跖肿胀度和AI显著升高(P<0.01),体质量显著下降(P<0.05)。(2)与模型组比较,新风胶囊组心脏指数(HI)、左室收缩期压(LVSP)和左室舒张期压(LV-EDP)显著降低(P<0.05,P<0.01),左室内压上升下降最大速率(±dp/dtmax)显著升高(P<0.05,P<0.01)。与甲氨蝶呤组比较,新风胶囊组LVSP、LVEDP显著降低(P<0.05),+dp/dtmax显著升高(P<0.05)。(3)与模型组比较,新风胶囊组TNF-α、脑利钠肽(brain natriuretic peptide,BNP)、IL-17显著降低(P<0.05,P<0.01),IL-10、CD+4、CD+4CD+25显著升高(P<0.05,P<0.01)。与甲氨蝶呤组比较,新风胶囊组IL-17显著降低(P<0.05),CD+4CD+25的表达显著升高(P<0.05)。(4)透射电镜显示新风胶囊组心肌超微结构基本完整,与正常组接近。结论 AA大鼠存在心功能下降和心肌超微结构破坏。新风胶囊在改善AA大鼠足跖肿胀度、关节炎指数的同时,也能改善其心功能,其机制可能与新风胶囊下调血清致炎因子水平,上调抑炎因子的表达,从而改善心肌细胞超微结构,保护心肌细胞有关。

Objective To observe the effects of Xinfeng Capsule （XC） on the cardiac function and the myocardial ultrastructure in rats with adjuvant arthritis （AA）. Methods Sixty rats were randomly divided into the normal control group, the model group, the methotrexate （MTX） treated group, the Tripterygium Glycosides Tablet （TGT） treated group, and the XC treated group, 12 in each group. Except those in the normal control group, rats in the rest groups were induced to establish the AA model by intradermally injecting Freund′s complete adjuvant into their right paws. The medication was started from the 19th day. Normal saline was administered to rats in the normal control group and the model group. MTX, TGT, and XC was respectively administered to rats in the MTX, TGT, and XC groups. The medication lasted for 30 days. The swelling degree of voix pedis, arthritis index （AI）, the heart function, serum levels of cytokines, and the myocardial ultrastructure were observed.Results （1） Compared with the normal control group, the swelling degree of voix pedis and AI significantly increased （P0.01）, the body weight significantly decreased （P0.05） in the model group and the other 3 treated groups. （2） Compared with the model group, the heart index （HI）, left ventricular systolic pressure （LVSP）, and left ventricular end diastolic pressure （LVEDP） significantly decreased （P0.05, P0.01）, the maximum rate of left ventricular pressure of development or decline （±dp/dtmax） significantly increased （P0.05,P0.01）. Compared with the MTX treated group, the LVSP and LVEDP significantly decreased （P0.05）, and ±dp/dtmax significantly increased （P0.05）.（3） Compared with the model group, TNF-α, brain natriuretic peptide （BNP）, and IL-17 significantly decreased （P0.05,P0.01）; IL-10, CD＋4, CD＋4CD＋25 significantly increased （P0.05, P0.01）. Compared with the MTX treated group, IL-17 significantly decreased （P0.05）, CD＋4CD＋25 expression significantly increased in the XC group （P0.05）. （4） Transmission electron microscopy showed that myocardial ultrastructure was basically contact in the XC treated group, approaching to that of the normal control group. Conclusions Decreased cardiac function and damaged myocardial ultrastructure existed in AA rats. XFC could ameliorate the swelling degree of voix pedis and AI, as well as improve the heart function. Its mechanisms might be correlated with down-regulating serum levels of inflammatory factors, up-regulating the expressions of anti-inflammation factors, thus improving the myocardial ultrastructure and protecting injured myocardial cells.