不同种类红豆杉愈伤组织的诱导及紫杉醇含量的差异

Differences in Callus Induction and Taxol Contents of Several Yew (Taxus L.) Species

研究云南红豆杉、东北红豆杉、红豆杉、南方红豆杉和杂种红豆杉形成愈伤组织的一些因素 ,比较不同植株来源的愈伤组织系的生长特点和紫杉醇含量。通过考察愈伤组织形成的时间和比率来研究取样时间、光照条件、培养基中 2 ,4- D浓度和外植体类型对愈伤组织诱导的影响 ,用高效液相色谱法测定愈伤组织中紫杉醇含量。愈伤组织形成的迟早和比率在不同种类红豆杉之间和同一种类不同植株之间差异较大。在黑暗中 ,在添加有 0 .1mg/ L BAP和 1.0～ 3.0 m g/ L 2 ,4- D的 B5 培养基上 ,5 0 %～ 10 0 %的云南红豆杉、红豆杉、南方红豆杉、杂种红豆杉和春、夏季取样的东北红豆杉的茎段可以形成愈伤组织 ;云南红豆杉和红豆杉愈伤组织系的紫杉醇含量普遍比较高 ,因此 。

Factors affecting callus induction of Taxus yunnanensis, T. cuspidata, T. chinensis, T. chinensis var. mairei and T. media were studied and the growth characteristics and taxol contents in the callus strains derived from different plants compared. Effects of sampling times, illumination, 2,4 D concentrations in medium and explant types on callus induction were studied by examining callus formation time and rate; HPLC method was used to determine taxol contents in callus cultures. 50%～100% of the stem segments of T. yunnanensis, T. chinensis, T. chinensis var. mairei, T. media and T. cuspidata sampled in spring or summer formed calli very well when they were cultured in dark on B 5 medium supplemented with 0 1 mg/L BAP and 1 0 mg/L 2,4 D. Generally, taxol contents in the callus strains of T. yunnanensis and T. chinensis were higher, so these two Taxus species can be used as the first choice explant source to obtain high taxol content callus strains.