红藻藻蓝蛋白对HL-60细胞生长的抑制作用

THE INHIBITION OF PHYCICYANIN FROM PORPHYRA HAITANENSIS ON THE GROWTH OF HUMAN LEUKEMIA HL-60 CELLS

本文运用半固体琼脂培养法及 MTT法研究了红藻藻蓝蛋白 (R- PC)对 HL- 60细胞生长的影响 ,结果发现 :R- PC能够显著抑制细胞的生长 ,且存在浓度效应和时间效应关系。为进一步探索 R- PC抑制 HL- 60细胞生长的可能机制 ,将不同浓度的 R- PC与 HL- 60细胞相互作用 6d后 ,分别用流式细胞仪检测 HL- 60细胞所处细胞周期中的时相和免疫印渍法测定 HL- 60细胞中 Bcl- 2基因表达产物的变化 ,实验结果显示 :R- PC确能使更多的 HL- 60细胞滞留于细胞周期的 G0 - G1 期 ,但与 R- PC浓度无正向关系 ,且 HL- 60细胞中 Bcl- 2基因表达产物的量也没有明显的变化。结果表明 :R- PC可能通过不同于 G0 - G1 期滞留及 Bcl- 2基因表达的机制抑制 HL- 60细胞的生长。

The effect of phycocyanin from Porphyra haitanensis on the growth of human leukemia HL-60 cells was studied by semi-solid culture method and MTT staining assay. The results showed that the phycocyanin could significantly inhibit the growth of HL-60 cells in a dose- an time-dependent manner. In order to elucidate the potential pathway of the phycocyanin affecting the growth of HL-60 cells treated with phycocyanin at different concentrations for 6 days were further checked by flow cytometric assay and western blot analysis. Flow cytometric assay demonstrated that more HL-60 cells underwent G 0-G 1 arrest,but there were not evident differences between the experimental groups and the control. Western bolt analysis indicated that the expression level of Bcl-2 protein remained unchanged.These findings suggested that the phycocyanin could inhibit the growth of HL-60 cells by the potential pathways other than G 0-G 1 arrested or the expression change of Bcl-2 protein.