iNOS在HBV相关肝细胞癌组织中的表达及对肿瘤血管形成及R0根治术后早期复发的意义

Expression of iNOS in the HBV related HCC tissue specimens affect angiopoiesis and the early recurrence of HCC patients after RO resection

目的:研究iNOS(诱导型一氧化氮合成酶)、VEGF、CD105在HBV相关肝细胞癌(HCC)组织中的表达和iNOS的表达与R0切除术后早期复发的关系。方法:应用组织芯片技术和免疫组织化学PV-6000两步法检测248例HBV相关HCC组织标本iNOS、VEGF和CD105的表达,应用RT-PCR检测-80℃低温冰冻HBV相关HCC标本20例iNOSmRNA的表达,分析iNOS的表达与VEGF、MVD-CD105的表达的关联以及与HCC患者R0根治术后早期复发的关系。结果:免疫组织化学法和RT-PCR均检测到iNOS HBV相关HCC组织中表达,iNOS阳性表达组中组织学分化呈中低分化者、血管癌栓和TNMⅢ-Ⅳ期者显著多于iNOS阴性表达组(P<0.05)。iNOS的阳性表达组中VEGF、MVD-CD105的阳性表达增高(P=0.032、0.020),iNOS阳性表达者其早期复发率高于阴性表达组(P=0.030),Cox回归分析表明iNOS阳性表达是HCC早期复发的独立影响因子。结论:在HBV相关HCC的患者组织中iNOS阳性表达与血管形成有关,iNOS阳性表达组肝外转移率高并且预示早期复发。

Objective： To study the expressions of iNOS,CD105,VEGF in the HBVrelated HCC tissue specimens and the relationships between them,the expression of the iNOS and early recurrence of the HCC after R0 resection.Methods： The expressions of iNOS,CD105,VEGF in the 248 HBVrelated HCC tissues were examined by tissue microarray and two-step methods of PV-6000 of immunohistochemistry and the expressions of iNOS in 20 frozen HCC specimens were examined by the RT-PCR and the correlations between them,and the expression of iNOS and the tumor early recurrence were analyzed.Results： The positive expression of iNOS in the HCC tissues was found in both immunohistochemistry and RT-PCR methods.The positive expression of iNOS was correlated with tumor early recurrence（P=0.000）,the expression of iNOS was one of the independent factors for the early recurrence by the cox regression analysis（P0.05）.In the iNOS positive expression group,the positive expression rate of CD105 and VEGF was significantly higher than in iNOS negative expression group（P=0.020,0.032）.Conclusions： The expression of iNOS is related with the tumor angiopoiesis,and the positive expression iNOS in HCC tissues implys the higher outside liver metastases rate,the early recurrence after R0 radical hepatectomy in the HBVrelated HCC patients.