陕产金柴胡总黄酮的含量测定及薄层鉴别

TLC Identification and Total Flavonoids Determination in Gold Radix Bupleuri Produced in Shaanxi Province

目的测定金柴胡中总黄酮含量,并对其中三个黄酮成分进行薄层鉴别。方法采用薄层色谱法对金柴胡药材中的芦丁、槲皮素、异鼠李素进行了定性鉴别;采用紫外分光光度法测定不同产地金柴胡中总黄酮的含量。结果薄层鉴别斑点清晰,重复性好,金柴胡中总黄酮平均含量为3.993%。结论该方法简便可行,重复性好,可作为金柴胡药材的质量控制方法。

objective To measure the content of total flavonoids in gold radix bupleuri, then TLC identification of three flavonoids ingredients had been conducted. Method TLC was used for the qualitative identification of Rutin, Quer- cetin and Isorhamnetin in gold radix bupleuri; Content of total flavonoids in from different producing areas was measured by UV spectrophotometry. Results The average content of total flavonoids in gold radix bupleuri was 3. 993 %. It can get the clear spot by TLC. Conclusions This method is simple, feasible, and reproducible, and can be used for the quality control of herbs gold radix bupleuri.