精索静脉曲张严重少精子症患者Y染色体微缺失分析

Y chromosome microdeletions in severe oligospermia men with varicocele

目的:探讨精索静脉曲张和Y染色体微缺失对生精障碍的影响。方法:对随机挑选的100例左侧精索静脉曲张严重少精子症患者(精子浓度<5×106/ml,组1),100例左侧精索静脉曲张轻度少精子症患者[精子浓度(10～20)×106/ml,组2],100例特发性严重少精子症患者(组3),100例特发性轻度少精子症患者(组4)和30例正常生育男性对照组(组5)采用聚合酶链反应(PCR)技术进行Y染色体微缺失检测,选取无精子因子(AZF)a、b和c区9个序列标签位点(STS)。结果:组1中有19例患者存在AZF微缺失(19%);组3中有11例患者存在AZF微缺失(11%),其余各组均未发现AZF微缺失;组1比组3有较高的缺失率。结论:在治疗精索静脉曲张严重生精障碍患者前应先进行Y染色体微缺失检测,避免不必要的治疗。

Objective： To investigate Y chromosome microdeletions in severe oligospermia men with varicocele. Methods： We randomly selected 100 cases of severe oligospermia with left varicocele （sperm concentration 〈5 × 106/ml, group 1 ）, 100 cases of mild oligospermia with left varicocele （sperm concentration 10 -20 × 106/ml, group 2）, 100 cases of idiopathic infertility with severe oligospermia （group 3 ）, 100 cases of idiopathic infertility with moderate oligospermia （group 4） and 30 normal fertile men as controls （ group 5 ）. We used polymerase chain reaction （PCR） technology to screen 9 sequence tagged sites （STS） of the AZF a, b and c re- gions and detect Y chromosome microdeletions. Results： AZF microdeletions were found in 19 patients in group 1 （ 19% ） and 11 in group 3 （ 11% ）, with a higher rate in the former than in the latter, but not in the other three groups. Conclusion ： Screening of Y chromosome microdeletions should be performed before the treatment of severe spermatogenesis with varicocele.