丙戊茶碱对慢性前列腺炎疼痛大鼠的作用及机制

Inhibitory effect of propentofylline on chronic prostatitis pain in rats and its mechanism

目的:探讨丙戊茶碱对慢性前列腺炎疼痛模型大鼠脊髓神经胶质纤维酸性蛋白(GFAP)及前列腺肿瘤坏死因子-α(TNF-α)的作用及机制。方法:将30只雄性SD大鼠随机均分为3组(n=10):假手术组(A组),模型组(B组),丙戊茶碱组(C组)。C组在造模后腹腔注射丙戊茶碱2 mg/kg,A、B组注射等量的生理盐水。用免疫组化法检测各组腰骶段脊髓神经胶质纤维酸性蛋白(GFAP)及前列腺肿瘤坏死因子-α(TNF-α)的含量。结果:A组GFAP(2.56±0.16)和TNF-α(1.34±0.05)的含量均低于B、C组;B组GFAP(16.79±0.72)和TNF-α(3.46±0.05)的含量均增加明显,与A组比较,差异有统计学意义(P<0.05);C组GFAP(8.83±0.63)和TNF-α(2.25±0.05)的含量增加幅度小,与B组比较,差异有统计学意义(P<0.05)。结论:丙戊茶碱可能通过抑制星形胶质细胞的激活及炎性介质的释放,对慢性前列腺炎疼痛大鼠模型的抑制发挥作用。

Objective： To investigate the effects of propentofylline on the expressions of glial fibrillary acidic protein （GFAP） and TNF-α and its action mechanism in the rat model of chronic prostatitis pain （CPP）. Methods： We equally randomized 30 male SD rats to groups A （ sham operation）, B （ CPP model） and C （ propentofylline intervention）. After modelling, the rats in group C received intraperitoneal injection of propentofylline at 2 mg/kg, while those in groups A and B were injected intrathecally with the same dose of normal saline. At 15 days after the treatment, we examined the expressions of GFAP in the spinal cord and TNF-α in the pros- tate by immunohistochemistry. Results： The levels of GFAP and TNF-α were obviously lower in group A （2.56 ± 0.16 and 1.34± 0.05） than in B （16.79 ± 0.72 and 3.46 ± 0.05） and C （8.83 ±0.63 and2.25±0.05）, significantly increased in B as com- pared with A （ P 〈 O. 05 ）. And the increase was markedly less in group C than in B （ P 〈 O. 05 ）. Conclusion ： Propentofylline in- hibits chronic prostatitis pain in the rat model by suppressing the activation of astroglia and the release of inflammatory mediators.