西妥昔单抗联合放疗对鼻咽癌细胞凋亡影响的研究

Enhancement of radiosensitivity by cetuximab in nasopharyngeal carcinoma

目的:西妥昔单抗(C225)对鼻咽癌细胞放疗增敏作用及其相关机制的研究。方法:通过免疫细胞化学法测得EG-FR在鼻咽癌CNE-2Z细胞株中的表达水平,MTT法测出西妥昔单抗在体外细胞实验中的工作浓度,克隆形成实验测定不同处理组的Do、SER值。将细胞分为对照组(N)、单纯照射组(R)、单纯用药组(C)和放疗联合使用西妥昔单抗组(RC)4组,应用流式细胞技术(Annexin V/PI标记)检测西妥昔单抗在体外对鼻咽癌细胞凋亡的影响。结果:免疫细胞化学法检测鼻咽癌CNE-2Z细胞株EGFR呈高表达水平。克隆形成实验测出放疗联合西妥昔单抗组Do低于单纯照射组,SER为1.581 1±0.035 7,P<0.05。放疗联合用药组的细胞凋亡率为(31.9±0.98)%,单纯放疗组的细胞凋亡率为(13.1±0.60)%,单纯用药组的细胞凋亡率为(6.4±0.95)%,对照组的细胞凋亡率为(2.5±0.51)%,放疗联合用药组的细胞凋亡率明显高于单纯处理组和对照组,P<0.05。结论:西妥昔单抗增加了鼻咽癌细胞对放疗的敏感性,其机制可能与其诱导细胞凋亡有关。

OBJECTIVE： To investigate the mechanism of enhancement of radiosensitivity by Cetuximab in nasopharyngeal carcinoma. METHODS： The expression of EGFR of nasopharyngeal carcinoma CNE-2Z cell line was tested by immunohistochemistry （IHC） and the working concentration of Cetuximab in vitro was detected by MTT colorimetry. Four groups were as follows： No treatment group（N）,Radiation alone group （R）,Cetuximab alone group （C）,Cetuximab after Radiation group （RC）. The working concentration were used to the four groups. The apoptosis-inducing effect of Cetuximab in vitro was detected via flow cytometry （Annexin V/PI labeled）. RESULTS： The expression of EGFR of CNE-2Z cell line was positive by IHC. The Do in Cetuximab and radiation group was lower than that in the radiation alone group. The SER of CR group was 1. 581 li0. 035 7 （P〈0. 05）. The apoptosis rate of radiotherapy and cetuximab group was （31.9±0. 98）% ,the radiation alone group was （13.1 ± 0. 60） %, the cetuximab alone group was （6.4±0.95） % and no treatment group was （2.5 ±0. 51） %. FCM showed more prominent apoptosis induced by cetuximab combined with radiation compared to cetuximab or radiation alone groups （P〈0.05）. CONCLUSION：Cetuximab can enhance the radiosensitivity of the CNE-2Z cell, which may be asso- ciated with apoptosis induction.