摘要
目的:观察胃癌组织血管内皮生长因子(VEGF)的表达和微血管密度(MVD),分析两者与胃癌临床病理学指标之间的关系。方法:即用型免疫组化EliVisionTMplus试剂盒检测80例胃癌组织VEGF及Ⅷ因子相关抗原的表达,Ⅷ因子相关抗原用作评估MVD的指标,分析VEGF和MVD与临床病理因素之间的相关性。结果:VEGF主要表达于癌细胞的细胞质中,阳性表达率为71.25%(57/80),VEGF的阳性表达与胃癌临床分期、TNM分期、是否有淋巴结转移和是否侵及浆膜密切相关,P<0.05。Ⅷ因子相关抗原表达于肿瘤间质血管内细胞胞质,胃癌组织MVD计数平均值为36.563±3.901。胃癌组织中MVD计数与胃癌是否有淋巴结转移、TNM分期、是否侵及浆膜和肿瘤大小相关,P<0.05。在57例VEGF阳性表达的胃癌组织中,MVD平均密度(36.83±8.87)高于在23例VEGF阴性表达的胃癌组织中的平均密度(31.08±9.54),差异有统计学意义,P<0.05。结论:VEGF的表达与胃癌的发生发展有密切关系,共同检测VEGF和MVD在胃癌组织中的表达可以作为判断胃癌患者预后的有效指标。
OBJECTIVE： To observe the expression and correlation of vascular endothelial growth factor （VEGF） and microvessel density （MVD） in gastric carcinoma tissues. METHODS： Immunhistochemical stain was used to test the expression of VEGF and Vi-R Ag. The difference of VEGF and Vi-R Ag expression among different indexs of pathologic factors was also analyzed. RESULTS： The positive rate of VEGF in 80 gastric cancer tissues was 71.25%（57/80）. VEGF expression was associated with clinical stage,TNM stage,lymph node metastasis and serosal invasion（P〈0.05）. The av- erage level of MVD in gastric cancer tissue was 36. 563±3. 901. The average level of MVD was also associated with TNM stage,lymph node metastasis and serosal invasion（P〈0.05）. The average level of MVD in gastric cancer tissues with VEGF positive expression（36.83 ±8.87）was higher than that of VEGF negative expression tissues（31.08 ± 9.54）. CON- CLUSIONS：The expression of VEGF may play a role in the tumor genesis and tumor development. VEGF and MVD may be a good marker to monitor the lymphatic metastasis of gastric carcinoma.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2012年第14期1078-1081,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
胃肿瘤
病理学
血管内皮生长因子
微血管密度
gastric neoplasms/pathology
vascular endothelial growth factor
microvessel density
