药对枳实-厚朴及其单味药中挥发油成分的比较分析

Comparative Analysis of Volatile Constituents in Herbal Pair Citrus Auruntium Fructus-Cortex Magnoliae Officinalis and its Single Herbs

比较分析药对枳实-厚朴及其单味药中挥发油成分。用气相色谱-质谱法(GC-MS)分离检测药对枳实-厚朴及其单味药的挥发油成分,通过交互移动窗口因子分析法(AMWFA)对其共有组分进行比较,结合质谱库、保留指数及总体积积分法进行定性定量分析。分别定性了枳实、厚朴、药对枳实-厚朴挥发油成分29,19和45种,分别占总含量的95.78%、81.01%、82.91%。药对与枳实、厚朴的共有组分分别为25和12种,三者的共有组分为5种。实验表明药对挥发油组分主要来自于单味药枳实。

The common volatile constituents in herbal pair（HP） Citrus Auruntium Fructus（CAF）-Cortex Magnoliae Officinalis（CMO） and its single herbs were comparison analyzed.Analysis of common volatile constituents in herbal pair（HP） and its single herbs was performed by the method of gas chromatography-mass spectrometry（GC-MS） and alternative moving window factor analysis（AMWFA） and by mass spectrometry library combining retention indices for qualitative and to quantitative by adopting total volume in integral method.In total of 25,19 and 45 volatile chemical components in volatile oil of CAF,CMO and HP CAF-CMO were separately determined qualitatively,accounting for 95.78%,81.01% and 82.91% total contents of volatile oil of CAF,CMO and HP CAF-CMO,respectively.The results indicated that there were 25 common volatile constituents in HP CAF-CMO and single herb CAF,and 12 common volatile constituents in HP CAF-CMO and single herb CMO,and 5 common volatile constituents among these three systems.The experimental results also showed that the volatile chemical components in HP CAF-CMO were mainly from single herb CMO.