大鼠非梗死区心肌Sos1/2蛋白表达的改变

Changes of Sos1/2 protein expression in non-infarcted myocardium in rats

目的:探讨大鼠非梗死区心肌Son of Sevenless(Sos)1/2蛋白表达的改变。方法:建立SD大鼠实验性心肌梗死(心肌梗死组)及假手术模型(假手术组),分别于术后24 h和12周处死2组大鼠各8只,获取心脏标本。应用免疫印迹法检测心肌梗死组中非梗死区心肌及假手术组心肌Sos1/2蛋白表达。结果:心肌梗死组术后12周心肌Sos1/2蛋白表达比假手术组术后24 h和术后12周及心肌梗死组术后24 h均显著增加[(0.40±0.13):(0.26±0.11):(0.24±0.08):(0.25±0.10),均P<0.05]。结论:心肌中存在Sos1/2蛋白表达。梗死后12周非梗死区心肌Sos1/2蛋白表达显著增加,提示Sos1/2蛋白表达增加参与了梗死后心脏的重塑。

Objective：To investigate the changes of Son of Sevenless（Sos）1/2 protein expression in non-infarcted myocardium in rats.Method：Experimental myocardial infarction（MI group） and sham-operation（sham group） models in SD rats were established respectively.Sos1/2 protein expression in non-infarcted myocardium in the MI group and in myocardium in the sham group was measured by Western blot respectively.Result：Sos1/2 protein expression in the non-infarcted myocardium in the MI group at 12th week after infarction [（0.40±0.13）] was significantly higher than those in the myocardium in the sham group at 24 h [（0.26±0.11）] and 12th week [（0.24±0.08）] after operation,and in the non-infarcted myocardium in the MI group at 24 h [（0.25±0.10）] after infarction respectively（All P0.05）.Conclusion：Sos1/2 protein expression exists in the myocardium in rats.The increased Sos1/2 protein expression can be involved in the left ventricular remodeling after infarction.