摘要
目的:探讨大鼠非梗死区心肌Son of Sevenless(Sos)1/2蛋白表达的改变。方法:建立SD大鼠实验性心肌梗死(心肌梗死组)及假手术模型(假手术组),分别于术后24 h和12周处死2组大鼠各8只,获取心脏标本。应用免疫印迹法检测心肌梗死组中非梗死区心肌及假手术组心肌Sos1/2蛋白表达。结果:心肌梗死组术后12周心肌Sos1/2蛋白表达比假手术组术后24 h和术后12周及心肌梗死组术后24 h均显著增加[(0.40±0.13):(0.26±0.11):(0.24±0.08):(0.25±0.10),均P<0.05]。结论:心肌中存在Sos1/2蛋白表达。梗死后12周非梗死区心肌Sos1/2蛋白表达显著增加,提示Sos1/2蛋白表达增加参与了梗死后心脏的重塑。
Objective:To investigate the changes of Son of Sevenless(Sos)1/2 protein expression in non-infarcted myocardium in rats.Method:Experimental myocardial infarction(MI group) and sham-operation(sham group) models in SD rats were established respectively.Sos1/2 protein expression in non-infarcted myocardium in the MI group and in myocardium in the sham group was measured by Western blot respectively.Result:Sos1/2 protein expression in the non-infarcted myocardium in the MI group at 12th week after infarction [(0.40±0.13)] was significantly higher than those in the myocardium in the sham group at 24 h [(0.26±0.11)] and 12th week [(0.24±0.08)] after operation,and in the non-infarcted myocardium in the MI group at 24 h [(0.25±0.10)] after infarction respectively(All P0.05).Conclusion:Sos1/2 protein expression exists in the myocardium in rats.The increased Sos1/2 protein expression can be involved in the left ventricular remodeling after infarction.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2012年第11期865-867,共3页
Journal of Clinical Cardiology
基金
重庆市卫生局医学科学技术研究项目(No:2009-2-290
04-2-154)
重庆市科委自然科学基金计划资助项目(No:CSTC 2007BB5276)
关键词
心肌梗死
Sos1
2蛋白
鸟嘌呤核苷交换因子
心肌
myocardial infarction
Sos1/2 protein
guanine nucleotide exchange factor
myocardium