一种新的继发性腹腔高压症大鼠模型的建立

Establishment of a new model of secondary intra-abdominal hypertension in rats

目的模拟严重失血性休克后过量复苏发生继发性腹腔高压症（intra—abdominal hypertension，IAH）的情况，建立一种稳定、可靠、低廉的大鼠IAH模型。方法16只成年雌性sD大鼠按随机数字表法分为假手术组和门静脉缩窄组，每组8只。门静脉缩窄组经腹分离门静脉，恒定缩窄；假手术组仅予以开关腹，分离门静脉。两组动物均采取腹部束缚，经股动脉插管放血致失血性休克，平均动脉压（MAP）维持40mmHg（1mmHg=0．133kPa）2h。回输全部失血及持续输入乳酸钠林格液的方法来建立SD大鼠的IAH模型。结果在门静脉缩窄组动物中除1只死于门静脉结扎后，其余动物均成功构建成大鼠IAH模型，成功率为87．5％，成功建模的时间为（4．68±0．53）h，成功建模的输液量为（597．33-4-75．03）ml/kg。假手术组动物无一只成功构建IAH模型。结论大鼠采用恒定程度的门静脉缩窄、腹部束缚方法，MAP维持于40mmHg2h后实施液体复苏至继发性IAH的模型，成功率高，重复性好。

Objective To create a stable, reliable and cheap rat model of intra-abdominal hy- pertension （IAH） by simulating the development of secondary IAH after overdose resuscitation for severe hemorrhagic shock. Methods Sixteen adult male SD rats were randomly divided into sham group and portal vein stenosis group, with eight rats in each group. Portal vein stenosis group had transabdominal separation of portal vein with constant stenosis, while sham group underwent simple laparotomy and dis- section of portal vein. Abdominal binding and hemorrhagic shock by drawing blood through the femoral ar- tery eannulation with mean arterial pressure （MAP） of 40 mm Hg （ 1 mm Hg =0. 133 kPa） for two hours were performed in two groups. Then, reinfusion of all autologous blood and continuous infusion of sodium lactate Ringer＇s solution were performed to induce IAH. Results In the portal vein stenosis group, IAH models were successfully built in all the rats except that one rat died after ligation of portal vein. The success rate of modeling was 87.5% , with total time of （4.68 ± 0.53 ） hours and total solution infusion of （597.33 ±75.03 ） ml/kg. No successful modeling was obtained in the sham group. Conclusion High success rate and good reproducibility of IAH models can be achieved by fluid resuscitation after con- stant portal vein stenosis, abdominal binding and hemorrhagic shock with mean MAP of 40 mm Hg for two hours.