Ganoderma lucidum U-281漆酶催化偶氮染料活性黑5脱色

Decolorization of reactive black 5 catalyzed by laccase from Ganoderma lucidum U-281

漆酶在纺织染料脱色及印染废水处理领域有着广阔的应用前景。活性黑5是纺织印染中应用广泛的偶氮类活性染料,结构复杂,生物降解性低。以灵芝菌Ganoderma lucidum U-281所产漆酶对活性黑5进行氧化脱色,采用单因素逐一优化方法得到了U-281漆酶催化活性黑5脱色的工艺参数:染料初始浓度25mg/L、漆酶用量2.0U/mL、铜离子添加量40mmol/L、pH6.0、40℃。在优化条件下,4h可使RB5脱色62.34%,24h可完成90%以上的脱色效果。

Laccase has an extensive application on the decoloration of textile dyes and waste-dyeing-water treatment. Reactive black 5 （RB5）, a representative azo dye, has complex structure and is low-biodegradable. In this paper, laccase produced by Ganoderma lucidum U-281 was used to decolorize RB5. The optimal parameters for RB5 decolorizing catalyzed by laccase from U-281 were seriatim obtained by single factor optimization method. The results were showed as follows： 25mg/L of initial dye concentration, 2.0U/mL of laccase dosage, 40mmol/L of CU2＋ addition and pH 6.0 at 40℃. Under the optimized conditions, 62.34% and more than 90% of RB5 was decolorized in 4 hours and 24 hours respectively.