摘要
目的:观察移植神经干细胞到可降解的聚乳酸/聚羟基乙酸(PLGA)神经导管修复大鼠面神经缺损的可行性。方法:20只SD大鼠随机分为2组,每组进行右侧面神经损伤手术,分别移植PLGA导管(A组)或含有神经干细胞的PLGA管(B组)。术后4、12周分别通过电生理检测、轴突形态学检测等方法来评估面神经的修复效果。结果:术后4周和12周,B组在神经动作电位的振幅分别为(27.53±7.48)、(141.23±21.18)μV,轴突的面积为(10 539.48±3 605.35)、(30 076.22±7 614.05)μm2,都高于A组[术后4周和12周,动作电位振幅为(13.03±11.38)、(118.50±22.70)μV;轴突面积为(6 633.20±3 759.30)、(20 772.53±10 903.65)μm2](P<0.05)。结论:神经干细胞移植可能会促进面神经的再生。
Objective：To construct a biodegradable PLGA nerve conduit（NC） filled with NSCs in order to improve facial nerve regeneration.Method：SD rats were subjected to right facial nerve transection,and PLGA NCs filled with NSCs were used to bridge the nerve gap.Facial nerve regeneration was assessed 4 and 12 weeks after surgery,through electrophysiological testing,and morphometric analysis of axons.Result：Nerve action potential amplitude,and axonal area were significantly greater in the NSCs group than the empty NC group.Conclusion：NSCs transplantation may improve regeneration of the facial nerve.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2012年第22期1040-1042,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
上海市卫生局青年基金项目(No:2009y013)
关键词
神经再生
移植
干细胞
神经导管
nerve regeneration
transplantation
stem cells
nerve conduit
