硫氧还蛋白对大鼠睾丸缺血再灌注损伤保护作用的研究

Protective effects of thioredoxin on ischemia/reperfusion-induced damaged testicular tissue after testicular torsion in rats

目的探讨硫氧还蛋白对大鼠睾丸缺血再灌注损伤的保护作用。方法选取雄性SD大鼠60只，随机分为4组：假手术对照组（A组）；睾丸扭转／复位组（B组）；睾丸扭转／复位＋腹腔内注射生理盐水组（C组）；睾丸扭转／复位＋腹腔内注射硫氧还蛋白组（D组）。无菌条件下制作左侧睾丸扭转模型，扭转持续4h，复位4h后取睾丸标本（D组在复位前15min腹腔内注射硫氧还蛋白）。对标本进行病理组织学检查；检测睾丸组织中超氧化物歧化酶（s0D）和丙二醛（MDA）的含量。结果睾丸扭转／复位后可见生精小管退变，间质出现水肿及出血，腹腔注射硫氧还蛋白使睾丸扭转／复位诱发的组织学改变明显改善。B组及C组睾丸损伤评分（8．3±0．96；8±0．87）明显高于A组（O．78±0．36）（P〈0．01），而腹腔注射硫氧还蛋白可以使睾丸损伤分值（3．1±0．42）显著降低（P〈0．05）。B组及C组MDA含量（4．39±0．21；4．42±0．17）升高，SOD活性（269±27．1；271±21．3）降低，与对照组（MDA：1．61±0．18；SOD：317±22．3）相比，差别具有显著性意义（P〈0．01）。而腹腔注射硫氧还蛋白能有效降低MDA含量（2．03±0．03）并升高SOD活性（315±24．2）（P〈0．01）。结论本实验为硫氧还蛋白作为治疗睾丸扭转继发损害的有效物质提供了组织学及生化依据。为临床预防和治疗睾丸缺血再灌注损伤提供了理论依据。

Objective The aim of this study was to examine the effectiveness of thioredoxin on the prevention of testicular damage induced by ischemia/reperfusion （I/R） in rats. Methods Male Sprague Dawley rats （n = 60） were divided randomly into 4 experimental groups：sham group, torsion- detorsion （TD） group, TD ＋ saline group, and TD ＋ thioredoxin group. Testicular ischemia was a- chieved by twisting the left testis for 4 hours, and reperfusion was allowed for 4 hours after detorsion. Human thioredoxin was injected intraperitoneally to rats in D group at time point of 15 min before detorsion. Left orchiectomies were harvested for tissue histopathological examinations. The detection of superoxide dismutase （SOD） activities and malondialdehyde （MDA） levels were also conducted. Re-suits Interstitial edema, dilatation, and degeneration of germ cells in tubules were observed after TD. Testicular tissues in D group showed an improved histological morphology. Testicular injury score in B group and C group （8. 3±0. 96; 8± 0. 87） were significantly increased compared with those in the Sham group （0. 78 ± 0. 36） （P〈0. 01）. It was observed that scores （3. 1 ± 0. 42） were significantly lower in D group compared with B and C groups（P〈0. 05）. The MDA concentration （4. 39 ± 0. 21; 4. 42±0. 17） and SOD activities （269±27.1;271 ± 21.3） in B and C group were increased and de-creased respectively as compared with the Sham group （MDA： 1.61 ± 0. 18; SOD： 317 ± 22. 3） （P〈 0. 0l）. Treatment of rats with human thioredoxin significantly reduced the tissue MDA levels （2. 03 ± 0. 03） （P〈0. 01）. SOD activities （315 ± 24. 2） were increased in testes in D group（P〈0. 01）. Conclu-sions The results provide biochemical and histopathological evidence for human thioredoxin to be used as a therapeutic agent for the testicular damage induced by I/R injury.