新疆地区维吾尔族及汉族溃疡性结肠炎患者人类白细胞抗原-DRB1等位基因与抗中性粒细胞胞质抗体的相关性

The correlation between human leukocyte antigen-DRB1 alleles and anti-neutrophii cytoplasmatic antiboies in Han and Uygur ulcerative colitis patients in Xinjiang region

目的研究人类白细胞抗原（HLA）-DRB1基因多态性与新疆地区维吾尔族及汉族溃疡性结肠炎（UC）患者抗中性粒细胞胞质抗体（ANCA）的关联性。方法用间接免疫荧光法分别对62例维吾尔族UC患者、58例汉族UC患者、188名维吾尔族健康对照者、184名汉族健康对照者进行啦清ANCA检测，采用聚合酶链反应-直接测序分型法（PCR-SBT）进行HLA-DRB1基因分型，分别在维吾尔族和汉族内比较ANCA阳性者、ANCA阴性者及健康对照者的HLA-DRB1等位基因频率；并按UC临床类型、严重程度、受累范围进行分层分析。应用SPSS17．0统计软件进行）（。检验，当P〈0．05时，计算比值比（OR）和95％可信区间（95％CI）。结果维吾尔族UC患者ANCA阳性率[53．2％（33／62）]高于汉族UC患者[34．5％（20／58）]，差异有统计学意义（χ2=4．269，P=0．045）。在维吾尔族中，ANCA阳性UC患者HLA-DRB1＊13的基因频率（0．202）显著高于ANCA阴性患者（0．017，χ2=10．092，P=0．016，OR=16．000，95oACI：2．892-88．524）和健康对照组（0．075，χ2=9．351，P=0．040，OR=3．407，95％CI：1．666～6．971）。ANCA阳性的全结肠炎型UC患者HLA-DRB1＊13基因频率（9／15）高于ANCA阴性的全结肠炎型UC患者（1／14），差异有统计学意义（χ2=8．955，P=0．040，OR=19．500，95% CI：2．787～136．461）。在汉族中，HLA-DRB1各等位基因在ANCA阳性者、ANCA阴性者和健康对照者间差异均无统计学意义（P均〉0．05），分层分析所获结果亦然。结论在新疆地区维吾尔族UC患者中，HLA-DRB1＊13可能与ANCA有关，还可能与全结肠炎型患者的ANCA有关。在新疆地区汉族患者中则可能无此关联性。

Objective To study the correlation between human leukocyte antigen （HLA）-DRB1 alleles and anti-neutrophil cytoplasmatie antibodies （ANCA） in Han and Uygur ulcerative colitis （UC） patients in Xinjiang region. Methods The serum ANCA was determined by indirect immunofluorescence assay in 62 Uygur UC patients, 58 Han UC patients, 188 Uygur and 184 Han healthy control individuals. HLA-DRB1 typing was performed by polymerase chain reaction-sequence based typing （PCR-SBT）. The allele frequency of HLA-DRB1 was compared in ANCA positive and negative.Han and Uygur patients as well as healthy controls. Stratified analysis was performedaccording to UC clinical type, severity and involvement. SPSS 17.0 software was applied for χ2 test. Once P〈0.05, the odds ratio （OR） and 95 %confidence intervals （95%CI） was calculated. Results The positive rate of ANCA in Uygur UC patients （53.2%, 33/62） was significantly higher than that of Han patients （34.5%, 20/58） and the difference was statistically significant （χ2 = 4. 269, P= 0. 045）. In Uygur, the gene frequency of HLA-DRB1 ＊ 13 in ANCA positive UC patients （0. 202） was significantly higher than that of ANCA negative patients （0. 017） （χ2= 10. 092, P=0. 016, OR= 16.000, 95%CI： 2.892 to 88. 524） and healthy controls （0.075） （χ2=9. 351, P=0.040, OR= 3.407, 95% CI： 1. 666 to 6. 971）. The gene frequency of HLA-DRB1 ＊ 13 in ANCA positive pancolitis type UC patients （9/15） was significantly higher than that of ANCA negative pancolitis type UC patients （1/14） and the difference was statistically significant （χ2 = 8. 955, P = 0. 040, OR = 19. 500, 95 %CI, 2. 787 to 136. 461）. However, in Han patients, there were no significant differences of HLA-DRB1 alleles frequencies among ANCA positive patients, ANCA negative patients and healthy controls （all P〈0.05）, and the results of stratified analysis were same. Conclusions In Uygur UC patients of Xinjiang region, HLA-DRB1 ＊ 13 may correlated with ANCA and with ANCA of pancolitis type UC patients. There is no such correlation in Han patients of Xinjiang region.