原发免疫性血小板减少症患者脾脏趋化因子受体CXCR3和CCR5的表达

Expression of CXCR3 and CCR5 chemokine receptor in spleens of patients with primary immune thrombocytopenia

目的探讨趋化因子受体CXCR3和CCR5在原发免疫性血小板减少症（ITP）患者脾脏中的表达及其临床意义。方法以10例ITP患者（ITP组）为研究对象，并以8例创伤性脾破裂患者脾切除标本作为正常对照。应用免疫组化法检测脾脏组织CXCR3、CCR5阳性表达率，用Westernblot法检测脾脏组织中CXCR3和CCR5的蛋白表达水平，逆转录聚合酶链反应检测CXCR3和CCR5mRNA表达水平。结果免疫组化结果显示ITP患者脾脏组织CXCR3、CCR5阳性表达率（90％、100％）均高于对照组（75％、87．5％），差异均有统计学意义（P〈0．05）；ITP患者脾脏组织CXCR3蛋白、mRNA表达量分别为对照组的3．0倍、3．5倍；CCR5蛋白、mRNA表达量分别为对照组的1．2倍、1．7倍，差异均有统计学意义（P〈0．05）。结论CXCR3及CCR5的高表达可能在ITP患者脾脏免疫紊乱中起着一定的作用。

Objective To study CXCR3 and CCR5 chemokine receptor expression in spleens of patients with primary immune thrombocytopenia （ITP） and its clinical significance. Methods The splenectomy specimens from 10 ITP patients （ITP group） and 8 patients with traumatic splenic rupture （normal control group） were studied. Immunohistochemistry （IHC） was used to study the positive rate of CXCR3 and CCRS. Western blot was performed to detect CXCR3 and CCR5 protein expression, while real-time polymerase chain reaction （RT-PCR） was conducted to analyze their mRNA expression. Results The positive rate of CXCR3 and CCR5 were both higher in ITP group （90% and 100%, respectively） than those in control group （75% and 87.5%, respectively） （P 〈0.05）. The differences were statistically significant （P 〈0.05）. Protein and mRNA level of CXCR3 in ITP group were 3.0 and 3.5 times as high as those in control group, respectively. Those of CCR5 in ITP group were 1.2 and 1.7 times as high as those in control group, respectively. Conclusion High expression of CXCR3 and CCR5 may play a part in the splenic immune disorders in patients with ITP.