葡萄糖调节蛋白78在PTSD样情感行为异常大鼠大脑皮质中的表达及意义

Expressions of GRP 78 in prefrontal cortex of rats with posttraumic stress disorder-like behavior

目的观察创伤后应激障碍(PTSD)大鼠大脑皮质内质网应激标志物葡萄糖调节蛋白78(GRP78)的表达变化,探讨内质网分子伴侣在PTSD发病机制中的作用。方法采用国际认定的SPS方法刺激建立大鼠PTSD模型,取成年健康雄性Wistar大鼠60只,随机分为SPS模型的1d、4d、7d组及正常对照组,采用免疫荧光法、免疫印迹和逆转录--聚合酶链式反应检测大鼠前额皮质GRP 78的表达变化。结果 SPS刺激后大鼠前额皮质神经元细胞内GRP78于1d开始逐渐升高,7d时表达最多;GRP 78mRNA的变化与之相一致。结论大脑皮质GRP 78的表达变化,可能是PTSD大鼠情感行为异常的重要病理生理基础之一。

Objective To observe the changes of GRP 78 in the prefrontal cortex neurons of PTSD rats may reveal part of the pathogensis. Methods The SPS-method was used to set up the rat PTSD mod- els. A total of 60 male Wistar rats were randomly divided into 1d,4d,7d groups of SPS and normal control group. The expression of GRP 78 were detected by using immunohistochemistry, western blotting and RT-PCR. Results The expression of GRP 78 in the prefrontal cortex gradually increased 1day after SPS, and reach the maximum at SPS 7d. The changes of GRP 78 mRNA was in accordance with the results of western blot. Conclusion The changes of expression of GRP 78 in prefrontal cortex of PTSD rats may play important roles in the pathogenesis of PTSD rats.