牙鲆遗传连锁图谱的构建

Construction of genetic linkage map in Japannese flounder(Paralichthys olivaceus)

利用基因组测序得到的大量微卫星序列,以681383B为父本、6812E36为母本杂交获得的F1为作图群体,构建了牙鲆(Paralichthys olivaceus)微卫星标记(SSR)遗传连锁图谱。雌雄图谱共定位SSR标记529个,其中雄性连锁图谱包括418个标记,分布在24个连锁群上,总长度1 418.1 cM,标记平均间隔3.62 cM,图谱覆盖率为88.7%;雌性连锁图谱包括437个标记,分布在24个连锁群上,总长度1 298.1 cM,标记平均间隔为3.16 cM,图谱覆盖率为89.1%。牙鲆中密度遗传图谱的构建为QTL分析以及分子标记辅助育种进一步奠定基础,并可以有效推动牙鲆的遗传改良工作,推动牙鲆养殖业的可持续发展。

Using the microsatellite sequence got from genome sequencing,we constructed genetic linkage map with SSR markers for Japanese flounder（Paralichthys olivaceus）,in which 681383B is the male parent and 6812E36 is the female parent.The male linkage map included 418 markers,which were distributed in 24 linkage groups,with 88.7% coverage.The length reached 1 418.1 cM,and the average marker interval was 3.62 cM.The female linkage map distributed in 24 linkage groups included 437 markers,with 89.1% coverage.spanning.The length reached 1 298.1 cM,and the average marker interval was 3.16 cM,.The medium density genetic linkage maps laid the groundwork for QTL analyses and marker-assisted selection breeding programs,and effectively promote the genetic improvement of Paralichthys,make flounder aquaculture sustainable development.