南极大磷虾粉对褶皱臂尾轮虫生长及脂肪酸组成的影响

Effects of Antarctic krill(Euphausia superba) powder on growth and fatty acids composition of rotifer Brachionus plicatilis

以活性干酵母培养的褶皱臂尾轮虫(Brachionus plicatilis)为对照,并以南极大磷虾粉对轮虫进行强化,设定轮虫培养液中南极大磷虾粉质量浓度分别为30、50和70 mg/L。分别测定强化12 h和18 h时轮虫的密度、带卵率以及强化后6 h、12 h、18 h轮虫的脂肪酸组成与含量。结果表明,12 h和18 h时,50 mg/L组的轮虫密度急剧升高,并显著高于其他3组,依次表现为50 mg/L组>30 mg/L组>对照组>70 mg/L组。带卵率方面:第12小时和18小时带卵率由高到低依次为50 mg/L组、30 mg/L组、70 mg/L组、对照组(P<0.05)。脂肪酸方面:6 h时,除了70 mg/L组的ARA与对照组、50 mg/L组和70 mg/L组的ω6-系列多不饱和脂肪酸总量(∑ω6-PUFA)与对照组没有显著差异外,其余强化组的ARA、EPA、DHA、ω3-系列多不饱和脂肪酸总量(∑ω3-PUFA)和∑ω6-PUFA都显著高于对照组(P<0.05);尤其是50 mg/L组的DHA和∑ω3-PUFA显著高于其他两个强化组和对照组(P<0.05),含量几乎达到对照组的10倍。12 h和18 h时,所有强化组的ARA、EPA、DHA、∑ω3-PUFA含量都显著高于对照组(P<0.05),而且50 mg/L组的上述脂肪酸含量都是最高的,或者与30 mg/L组和70 mg/L组并列第一。3个强化组的DHA/EPA比值、EPA/ARA比值也显著高于对照组(P<0.05)。结论认为,南极大磷虾粉的强化剂量以50 mg/L为优,强化时间则以12 h和18 h为宜,南极大磷虾粉对褶皱臂尾轮虫具较好的营养强化效果。

Rotifer Brachionus plicatilis was fed with dry yeast（control） and different Antarctic krill powder dos-ages（30 mg/L,50 mg /L and 70 mg/L）.The effect of Antarctic krill powder enrichment were assayed through its individual density and egg ratio at feeding time of 12 h and 18 h,and fatty acid content at feeding time of 6 h,12 h and 18 h,repectively.The results showed that： at 12 h and 18 h,individual density in 50 mg/L group dramatically increased and was significantly higher than other three groups（P0.05）,followed by 30 mg/L group,control group and 70 mg/L group,successively.Egg canrying ratio also varied among dietary treatments at 12 h and 18 h,and in group of 50 mg/L which was the highest,followed by 30 mg/L and 70 mg/L,successively,and control group was the lowest（P0.05）.In terms of fatty acids at 6 h,the ARA,EPA,DHA,∑ω3-PUFA and ∑ω6-PUFA contents in the groups fed with Antarctic krill powder were significantly higher than those in the control group（P0.05）,except for ∑ω6-PUFA in 50 mg/L group,and ARA,∑ω6-PUFA in 70 mg/L group.The highest contents of DHA and ∑ω3-PUFA were found in 50 mg/L group with a level of 10 times higher than that of the control group at 6 h.At 12 h and 18 h,the amount of ARA,EPA,DHA and ∑ω3-PUFA were significantly higher than that of the control group（P0.05）,and the highest amount of these fatty acids were observed in 50 mg/L group.Also,DHA/EPA and EPA/ARA were significantly great for treatment groups compared with control group（P0.05）.Generally,the 50 mg/L group had a better effect for increasing fatty acid content compared to the other three doses,and the suitable duration for increase fatty acid content was 12-18 h.In conclusion,Antarctic krill powder has a good effect on strengthening the nutritional composition in rotifer B.plicatilis.