CD105和细胞间粘附分子-1在胃癌中的表达及意义

Expression and clinical significance of CD105 and ICAM-1 in gastric cancer

目的研究CD105在胃癌新生血管中的表达及胃癌患者胃癌组织及外周血中细胞间粘附分子-1(ICAM-1)的表达,探讨其在胃癌生长、转移和判断预后中的临床价值。方法以鼠抗人CD105单克隆抗体应用免疫组织化学染色法检测胃癌及正常组织中的微血管密度(MVD),采用免疫组织化学染色和酶联免疫吸附法分别检测胃癌患者胃癌组织和外周血中ICAM-1的水平。结果 MVD和ICAM-1在胃癌组明显高于对照组,在胃癌组中MVD和ICAM-1与浸润深度、淋巴结转移和TNM分期有关(P<0.05或<0.01),和肿瘤分化程度无关(P>0.05)。结论以CD105标记的MVD和ICAM-1可作为反映胃癌生物学行为的客观指标,为临床判断胃癌的生长、转移、分期及预后提供一定的依据。

Objective To investigate the expression of CD105 in neovessels of gastric cancer and the expression of ICAM-1 in gastric cancer tissues and peripheral blood of patients with gastric cancer in order to explore their clinical significance in the growth, metastasis and prognosis of gastric cancer. Methods The microvessel density （ MVD ） in gastric cancer tissue and normal tissue was detected by immnnohistoehemistry staining by means of CD105 monoclonal antibody, and the expression levels of ICAM-1 in gastric cancer tissues and peripheral blood of patients with gastric cancer were detected by immunohistochemistry staining or enzyme linked immunosorbent assay （ELISA）, respectively. Results The MVD and expression levels of ICAM-lin gastric cancer tissues were significantly higher than those in normal tissues, and MVD and expression levels of ICAM-1 were correlated with the depth of invasion, lymphatic node metastasis and TNM staging （ P 〈 0. 05 or 〈 0. 01 ）, however,which were not related to the differentiation degree of tumor （ P 〉 0.05 ）Conclusion MVD marked by CD105 and ICAM-1 can be used as the objective indexes to reflect the biological behavior of gastric carcinoma, which can provide certain basis for evaluating tumor＇ s growth, metastasis, staging and patients＇ prognosis.