张家口地区仔猪黄痢病原菌血清型检测及药敏试验

Investigation on the Serotype and Antibiotic Resistance of E.coli Isolated from Piglit's Yellow Dysentery in Large-scale Pig Farms in Zhangjiakou

为了调查张家口地区仔猪黄痢病例大肠杆菌的耐药性及流行血清型,从发生仔猪黄痢的20个规模化猪场采集90份病料,进行致病性大肠杆菌的分离及生化鉴定。腹腔接种小白鼠以鉴定分离菌的毒力,采用玻板凝集试验和试管凝集试验鉴定分离菌O血清型,并使用16种抗菌药对50株致病性大肠杆菌进行药敏试验。结果表明,从90份病料中分离出86株大肠杆菌,其中致病性大肠杆菌79株。79株致病菌共有10种血清型,其中O8、O151、O149、O141为优势血清型,占能定型分离致病菌株的61.1%。药敏试验结果显示,头孢噻肟钠抑菌作用最强(94%),其次为诺氟沙星(90%)、丁胺卡那霉素(82%)、新霉素(80%)。50株大肠杆菌对16种抗菌药均有不同程度的耐药性,对链霉素完全耐药,其中39株为5耐以上的菌株,占供试菌株的78%。

In order to investigate the antibiotic resistance and predominant serotypes of E. coli of yellow dysentery, a total of 90 fecal samples were collected from 20 large-scale pig farms for isolation and identification of E. coli. The virulence of the isolates was tested in mice and the sero- type was tested by the agglutination. Finally,a total of 86 E. coli isolates were obtained and 79 of them were tested to be pathogenic,among which 72 isolates were serotyped to belong to 10 kinds of serotypes and the predominant serotypes were O8, O151, O149 and O141. Among all the iso- lates,50 isolates were resistant to 16 kinds of antibiotics to a greater or less degree. The highest resistance to streptomycin was 100%. The efficient antibiotics were cefotaxime sodium, norfloxa- cin,amikacin and neomycin,with the sensitive ratio of 94%（47/50）,90%（45/50）,82%（41/50）,and 80%（40/50）,respectively. All the 50 isolates showed resistance to more than 5 kinds of anti- biotics.