正交试验法优选复方丹参片多糖的提取工艺

Optimization of Extraction Technology for Polysaccharides in Compound Danshen Tablets by Orthogonal Design Method

目的优选复方丹参片多糖的提取工艺。方法采用超声法、设置四因素三水平正交实验提取复方丹参片多糖。结果最佳提取方案为:料水比=1:40;提取时间35 min;醇沉浓度为乙醇浓度达90%;提取次数1次。结论通过正交试验确定的最佳方案,多糖提取量稳定,方法简单、可行。

Objective To optimize extraction technology for polysaccharides in Compound Danshen Tablets. Methods Ultraphonic method at 60℃, orthogonal experiments with four factors and three levels were designed to optimize the extraction of Compound Danshen Tablets polysaccharides. Results The optimum extraction conditions were as follows： Compound Danshen Tablets to water=1：40; extraction time 35 min; ethanol concentration 90%; extraction once. Conclusion The amount of the extraction ofpolysaccharides was stable with the optimum scheme, the method was simple and feasible.