摘要
目的建立测定安神颗粒中酸枣仁皂苷A及B含量的方法,提供该制剂质量控制标准。方法应用高效液相色谱法-蒸发光散射检测器(HPLC-ELSD),Kromasil C18色谱柱(4.6 mm×200 mm,5μm),流动相:甲醇-水-冰醋酸(65:35:1),流速:1.0 mL/min,柱温25℃,氮气流速2.0 L/min,漂移管温度94℃,测定安神颗粒中酸枣仁皂苷A和B含量。结果酸枣仁皂苷A及B平均回收率分别为100.2%(RSD=1.21%)和99.88%(RSD=1.32%)。结论此方法操作简便,结果准确、可靠,可有效控制制剂的质量。
Objective To establish a method for the determination ofjujuboside A and B in Anshen Granules and to provide the quality control standard for preparation. Methods Jujuboside A and B in Anshen Granules were determined by high performance liquid chromatography-evaporative light-scattering detector（HPLC-ELSD）. The column was Kromasil C18 （4.6 min× 200 mm,5μm） and the mobile phase consisted of methanol-water-glacial acetic acid （65：35：1） with the flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the nitrogen flow rate was 2.0 L/min and the temperature of the drift tube was 94℃. Results The average recovery ofjujuboside A and B were 100.2 % （RSD 1.21%）和99.88 % （RSD 1.32 %） , respectively. Conclusion The method is simple, accurate and reliable for the quality control of Anshen Granules.
出处
《食品与药品》
CAS
2012年第11期434-436,共3页
Food and Drug
