自体骨髓在兔腰椎横突间融合中诱导成骨的组织学评估

The histological evaluation of the effect of autologous bone marrow in osteogenous inducement in lumbar intertransverse spinal fusion in rabbits

目的研究自体骨髓在兔腰椎横突间融合中的诱导成骨作用。方法骨骼成熟的中国白兔24只,行双侧腰5～6椎体横突间植骨融合。实验组除植入髂骨外,取2ml自体骨髓植入融合区。不同时期处死动物,脊柱标本应用组织学方法分析。结果各时期组织学检查显示:实验组和对照组标本切片中均可见到膜内成骨和软骨成骨两种成骨方式,实验组标本中膜内成骨明显占优势,对照组则以软骨成骨为主,此组切片中可见到更多的纤维组织。结论兔腰椎横突间融合模型中,自体骨髓是一种有效可靠的骨移植添加物,可在应用有限自体髂骨植骨的情况下,获得更好的融合效果。

Objective To investigate the effect of autologous bone marrow augmentation in osteogenous inducement in lumbar intertransverse spinal fusion in rabbits.Methods 24 Chinese white rabbits with skeletal maturation（3.0~3.5kg） were enrolled in the study.A single-level posterolateral intertransverse process lumbar spine fusion was performed at L5~6,and corticocancellous bone graft was placed equally in the each side of intertransverse process bed.The 2ml autogenous bone marrow in experimental group was taken out from another iliac crest.The rabbits were killed after 4,8 and 12 weeks,and the spine specimens were excised and analyzed by histological method.Results Intramembranous ossification and endochondral ossification could be observed at different time points both in experimental group and control group.The intramembranous ossification was predominant in experimental group,however,more endochondral ossification was found in control group,with more fibrous tissues showed in sections.Conclusion Autogenous bone marrow is a reliable and effective bone transplantation extender in the animal model of posterolateral intertransverse lumbar spine fusion in rabbits,which can acquire preferable fusion result,with the application of limited autoallergic iliac bone graft..