摘要
根据GenBank中拟南芥RAX基因序列,在烟草EST数据库中进行Blastp检索,获得与之同源性较高的蛋白质序列,选取同源性最高的蛋白质序列所对应的核酸序列,设计引物,获得中间片段,再运用中间片段设计引物,应用RACE方法获得其5′和3′末端cDNA序列,将获得的3段序列拼接,结合全长克隆测序验证,获得一个新的普通烟草基因,将其命名为NtRAX(NCBI登录号为JQ228591)。NtRAX的cDNA全长为1112 bp,5′端非编码区96 bp,3′端非编码区62 bp,编码区长度为954 bp,编码317个氨基酸,预测的分子量是35.42 KDa。对该基因进行序列分析和功能预测,得出该基因属于MYB基因家族,是具有转录激活活性的烟草腋芽分生组织形成的调控基因。
Based on Arabidopsis RAX gene sequence in GenBank,we conducted a Blastp search in tobacco EST database,and acquired a high homology with the protein sequence,then selected the highest protein sequence homology to the corresponding nucleic acid sequences to design primers,obtained the middle fragment,then used it to design primers,obtained the 5 ＇and 3＇ ends of the cDNA sequence by RACE strategy,a gene named NtRAX（NCBI accession number JQ228591） was obtained by splicing three cDNA sequences and sequencing full-length cloning.The full-length cDNA of NtRAX is 1112bp long with 96bp 5′-UTR,62bp3′-UTR and 954bp coding sequence.The deduced amino acid is 641 AA long.Predicted molecular weight is 35.42KDa.NtRAX was predicted a tobacco axillary meristem formation regulatory genes with transcriptional activation activity,belonged to the MYB gene family.
出处
《中国烟草科学》
CSCD
2012年第5期14-18,共5页
Chinese Tobacco Science
基金
中国农业科学院基本科研业务费项目“烟草腋芽基因的RNA干扰研究”(0032011022)
