摘要
目的探讨瘦素对大鼠关节软骨细胞转化生长因子-β1(TGF-β1)表达的影响。方法培养大鼠关节软骨细胞,按实验方法将瘦素(浓度为10ng/ml)作用条件下培养的软骨细胞作为实验组、将单纯培养的软骨细胞作为对照组;于不同时间(12、24、36、48、72h)检测2组TGF-β1水平。结果 (1)采用细胞体外培养方法成功培养出大鼠关节软骨细胞,并通过形态观察和特殊染色得到证实。(2)实验组TGF-β1基因表达明显高于对照组,最佳时间为作用24～48h;继续瘦素刺激细胞增殖不明显,并有抑制作用的趋势。结论 (1)低浓度瘦素可显著提高软骨细胞的活性;(2)低浓度瘦素能有效维持大鼠关节软骨细胞的表型,抑制软骨细胞"去分化"和退变,并通过上调TGF-β1的mRNA表达促进细胞外基质II型胶原和蛋白多糖的合成分泌;(3)瘦素可加强TGF-β1的mRNA表达,以防止细胞外基质降解,有修复软骨的潜能。
Objective To discuss the impact of leptin on the expression of articular chondrocyte transforming growth factor-β1（TGF-β1） of rat. Methods Cultured rat articular chondrocyte, by experimental method, leptin （concentration of 10ng/ml） role under the condition of cultured cartilage cell were as experimental group, the pure cultured chondrocyte were as control group;At different time（ 12,24,36,48,72h） ,detected the level of TGF-β1 of 2 groups. Results （ 1 ）By cell cultured in vitro method successfully cultured rat articular chondrocyte, and was confirmed by morphological observation and special staining. （2）The TGF-β1 gene expression of experimental group was significantly higher than that in control group, the best time for role 24 to 48h;Continue leptin to stimulate cell proliferation was not obvious,and had a trend of inhibition role. Conclusion （ 1 ） Low concentration of leptin may significantly increase the activity of the cartilage cell; （2） Low concentration of leptin can effectively maintain the phenotype of rat articular chondrocyte, inhibition of chondrocyte dedifferentiation and degeneration, and by up-regulating TGF-β1 mRNA expression for type II collagen and proteoglyean synthesis and secretion of extracellular matrix;（ 3 ）Leptin may enhance TGF-β1 mRNA expression, in order to prevent the degradation of extracellular matrix, has the potential to repair cartilage.
出处
《临床合理用药杂志》
2012年第34期23-24,共2页
Chinese Journal of Clinical Rational Drug Use
