摘要
目的建立同时测定铁皮石斛制剂中12种农药残留量的分析方法。方法样品用乙腈超声提取,经过石墨化碳/氨基固相萃取柱净化,用高效液相色谱-质谱串联法测定,采用内标法定量。结果方法回收率72.1%～119.5%,RSD均<15%。各农药检测限均<0.001 mg.kg-1。结论该方法简单,快速,灵敏,结果准确,重复性好,可用于铁皮石斛制剂12种农药残留量的测定。
Objective To establish a method for the simultaneous determination of 12 kinds of pesticide residues in the Dendrobium officinale prescription.Methods The total of 12 pesticides was extracted by ultrasonic,and then purified through Carb/NH2 solid phase extraction（SPE） cartridges.The residues were simultaneous identified and quantified by HPLC/MS/MS.Results The recoveries ranged from 72.1%~119.5%,with their relative standard deviations（RSDs） being less than 15%.The limit of detection（LOD） for each pesticide was below 0.001 mg·kg-1.Conclusion This method was simple,sensitive and reproducible,which could be appilied to determine 12 kinds of pesticide residues in Dendrobium officinale prescription.
出处
《医药导报》
CAS
北大核心
2012年第11期1481-1484,共4页
Herald of Medicine
