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RNA干扰的研究现状与应用前景 被引量：4

The Research Status and Application Prospect of RNA Interference
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摘要 RNA干扰(RNAi)是dsRNA介导的特异性基因表达沉默的现象,是生物在进化上高度保守的基因表达调节机制之一。从RNA干扰的概念入手,系统阐述了RNA干扰的作用机制、作用特点及其最新进展,并对RNA干扰技术的应用前景进行了展望。 RNA interference （RNAi） is an evolutionarily conserved mechanism of gene regulation in almost all living organisms, with roles ranging from the developmental regulation of gene expression to defense mechanism against viral infections. In this paper, the concept, mechanism, basic characteristics and new research progress of RNA Interference are summarized. At the end, the application prospects of RNA Interference are described.
作者 陈贵元 谭德勇
机构地区 云南大学生命科学院生物化学与分子生物学实验室 大理学院基础医学院生物化学与分子生物学教研室
出处 《大理学院学报（综合版）》 CAS 2012年第10期52-55,共4页 Journal of Dali University
基金 国家自然科学基金资助项目(30760057 30960091)
关键词 RNA干扰机制 作用特点 应用前景 RNAi mechanism function characteristics application prospect
分类号 R979.1 [医药卫生—药品] R978 [医药卫生—药品]
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参考文献15

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同被引文献63

  • 1邹丰才,宋慧群,陈宁,朱兴全.RNA干扰技术及其在寄生线虫基因功能研究中的应用[J].中国兽医科学,2006,36(1):80-84. 被引量：3
  • 2王甦,张润志,张帆.异色瓢虫生物生态学研究进展[J].应用生态学报,2007,18(9):2117-2126. 被引量：111
  • 3Caplen N J, Parrish S, Imani F,et al. Specific inhibition of gene expression by small double-stranded RNAs in inverte- brate and vertebrate systems[J]. Proceedings of the National Academy of Sciences of the United States of America, 2001,98 (17):9 742-9 747.
  • 4Elbashir S M, Lendeckel W, Tuschl T. RNA interference is mediated by 21-and 22-nucleotide RNAs[J]. Genes- Develop- ment, 2001,15(2) : 188-200.
  • 5Kuan-Teh Jeang. RNAi in the regulation of mammalian viral infections[J]. Jeang BMC Biology, 2012,10 : 58.
  • 6Okamura K, Lai EC: Endogenous small interfering RNAs in animals[J]. Nat Rev Mol Cell Biol, 2008,9 : 673-678.
  • 7Golden D E, Gerbasi VR, Sontheimer E J. An inside job for siRNAs[J]. Mol Cell, 2008,31 : 309-312.
  • 8Rother S,Meister G. Small RNAs derived from longer non-cod- ing RNAs[J]. Biochimie,2011, 93:1 905-1 915.
  • 9Jiangnan Li,Yajuan Dai,et al. In vitro inhibition of CSFV rep- lication by multiple siRNA expression[J]. Antiviral Research, 2011,91:209-216.
  • 10Ke Lv, Yingjun Guo, Yiliang Zhang, et al. Transient inhibition of foot-and-mouth disease virus replication by siRNAs silen-cing VP1 protein coding region[J]. Veterinary Science, 2009, 86:443-452.

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大理学院学报（综合版）
2012年 第10期

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