高效分子排阻色谱-蒸发光散射检测法测定右旋糖酐分子量及其分布

Determination of molecular weight and distribution of dextran by HPSEC-ELSD

目的建立高效分子排阻色谱-蒸发光散射检测法,用于测定右旋糖酐40的分子量及其分布。方法采用TSK-gel G4000PWXL色谱柱(10μm,7.8 mm×300 mm),流动相为水,流速0.6 ml/min,柱温30℃,蒸发光散射检测器。结果右旋糖酐平均重均分子量在4 600～133 800 u范围内线性关系良好(R2=0.991),精密度试验相对标准偏差(RSD)为0.252%,重复性试验RSD为0.460%。结论该方法灵敏度高,重复性好,可作为右旋糖酐40的分子量及其分布测定的分析方法。

Objective To set up a method for determination of molecular weight and distribution of dextran 40 by high performance size exclusion chromatography（HPSEC） with evaporative light scattering detector（ELSD）.Method The TSK-Gel G4000 PWXL（10 μm,7.8 mm×300 mm） column was applied with water as mobile phase.The flow rate was 0.6 ml/min,and column temperature was set at 30℃.Results As for the average molecular weight of dextran,the linear range was between 4 600~133 800 u（R2=0.991）.Relative standard deviations（RSD） of precision and repeatability were 0.252% and 0.460% respectively.Conclusion The method is accurate with high sensitivity which could be applied for the measurement and quality control of molecular weight and distribution of dextran 40.