绿萝组织培养与快繁技术

Tissue culture and rapid propagation technologies for Scindapsus aureus

【目的】研究绿萝组培快繁技术要点,为绿萝再生体系建立和工厂化生产提供技术支持。【方法】以绿萝叶片和无节茎段为外植体,以MS为基本培养基,探讨不同浓度的激素组合(1.0～3.0mg/L6-BA、0.1～0.3mg/LNAA、0.1～0.3mg/LIBA)对愈伤组织诱导及其分化、不定芽诱导、生根培养等的影响。【结果】两种外植体均能产生愈伤组织,但茎段产生愈伤组织较快,产生不定芽也快。叶片诱导愈伤组织的最适培养基为MS+6-BA3.0mg/L+NAA0.3mg/L,愈伤组织分化的最佳培养基为MS+6-BA2.0mg/L+NAA0.2mg/L。茎段诱导愈伤组织及其分化的最适培养基均为MS+6-BA2.0mg/L+NAA0.2mg/L,最适生根培养基为1/2MS+NAA0.2mg/L。【结论】绿萝叶片和茎段可作为愈伤组织培养的较好外植体,茎段培养效果优于叶片。

[Objective]This research explored the tissue culture and rapid propagation of Scindapsus aureus in order to provide technical supports on green regeneration system establishment and factory production. [Method]The leaf and stem without buds of S. aureus were used as explants to investigate the effects of different plant hormone combinations （1.0-3.0 mg/L 6-BA, 0.1-0.3 mg/L NAA, 0.1-0.3 mg/L IBA） added to MS culture medium on callus induction, differentiation, adventitious bud, and rooting culture. [Result]The results showed that the explants could effectively induce the callus formation, but the stems induced callus and produced adventitious buds quickly. The suitable culture medium for leaves to induce callus was MS＋6-BA 3.0 mg/L＋NAA 0.3 mg/L, and the best euhure medium of callus differentiation was MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L. For stem without buds, the best medium for callus induction and differentiation was MS＋6-BA 2.0 mg/L＋NAA 0.2 rag/L, and the optimum culture medium for rooting culture was 1/2MS＋NAA 0.2 mg/L. [Conclusion]Leaf and stem without buds of Scindapsus aureus were better explants for callus culture, and stem show bet- ter culture effects than leaves.