海南粗榧内生真菌细极链格孢CH1307产高三尖杉酯碱的发酵条件

Optimization of Homoharringtonine Fermentation Conditions for Alternaria tenuissima CH1307,an Endophytical Fungus of Cephalotaxus mannii Hook.f.

以海南粗榧内生真菌细极链格孢CH1307发酵产高三尖杉酯碱的产量为指标,采用响应曲面法分析葡萄糖质量浓度、硫酸镁质量浓度、复合维生素B质量浓度及摇床转速对该菌株液态发酵的影响。结果表明,细极链格孢CH1307液态发酵产高三尖杉酯碱的关键因子值分别为葡萄糖32.0226g·L-1,硫酸镁0.0966g·L-1,复合维生素B0.1239g·L-1,转速146.4742r·min-1时CH1307发酵最好,代入方程的HHRT产量为190.6370μg·L-1。

Homoharringtonine （HHRT） was produced from Alternaria tenuissima CH1307, an endophytical fun- gus of Cephalotaxus mannii Hook. f through fermentation. Four factors such as the concentrations of glucose, magnesium sulfate and vitamin B compound and the rotating speed were analyzed by using response surface methodology to determine their effect on fermentation of the fungus CH1370. The best operating conditions resul- ted to be 32.022 6 g · L-1 for glucose , 0. 096 6 g·L-1 for magnesium sulfate and 0. 123 9 g ·L-1 for vitamin B compound as well as 146. 474 2 r · min-1 for the rotating speed, and 190.637 0 μg·L-1 of HHRT were pro- duced from this fungus when the values of the four factors were entered into a formula established.